U-Taq DNA Polymerase is a robust thermostable Taq enzyme that can withstand prolonged incubation at temperatures up to 95°C without significant loss of activity.
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U-Taq DNA Polymerase is a robust thermostable Taq enzyme that consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5'→3' DNA polymerase activity and lacks 3'→5' exonuclease activity. Its extension rate is 2~4 kb/min in standard conditions, which is the highest among all thermostable DNA enzymes. The appropriate reaction temperature is 70~75℃, the work concentration of dNTPs is 100~300 μM, the work concentration of Mg2+ is 2~3 mM, and the suitable pH is 8.1~9.1. The enzyme generates PCR products with 3'-dA overhangs, suitable for T-A cloning. The amount of enzyme is 1~1.5 units for 20μl PCR reaction, while 2~3 units for 50μl PCR reaction. 6 kb Lambda DNA and 2.1 kb Human genomic DNA can be amplified very well at our laboratory.
U-Taq DNA Polymerase Storage Buffer: U-Taq DNA Polymerase is supplied in 50 mM Tris-HCl (pH8.0), 100 mM NaCl, 0.1 mM EDTA,0.5 mM DTT, 1% TritonX-100, and 50% Glycerol.
10 x U-Taq reaction buffer: 500 mM KCI, 100 mM Tris-HCI (pH8.0), 20 mM MgCI2.
Store at -20°C for one year. Avoid repeated freeze-thaw cycles
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The PCR reactions contained 1X PCR buffer, 2.5 mM MgCl2, 0.4 mM of each dNTP, 0.4 μM of each primer, 1 U Taq polymerase (SBS Genetech Co., Ltd., China), and 1 μL of template DNA (100 ng μl−1). The PCR reaction was adjusted to a final volume of 25 μl with MQ water.
Sequencing of Norovirus in Southern, Nigeria: Prevalent Genotypes and Putative GII.4 Novel Recombinants among Children
The RT-PCR used is a very sensitive method, it can detect as few as 5 x 106 copies per gram of stool sample. U-TaQ DNA polymerase (SBS genetech, Beijing, China), a high-fidelity thermostable enzyme that can withstand prolonged incubation at high temperature up to 95°C without significant loss of activity was used for this RT-PCR protocol.
Semi-nested polymerase chain reaction over blood culture in detection of bloodstream fungal infection in leukemic children with febrile neutropenia
Total RNA from HepG2 cells was isolated using the RNeasy mini kit (QIAGEN) following manufacturer’s instructions. Retrotranscription was performed using 2.5 μg of RNA, a specific HSA oligonucleotide (ATAAGCCTAAGGCAGCTTGACTGG) and Superscript II reverse transcriptase (Invitrogen). The full-coding sequence of HSA was PCR- amplified with U-Taq (SBS GeneTech)
Evaluation of Nested broad-range PCR for Pathogen Detection in Negative Blood Cultures
La reacción fue puesta a punto en un volumen final de 25µL conteniendo: 100ng de ADN genómico, 2.5µL de Buffer de PCR 10X (Mg2+: 20mM), 1µM de cada primer, 10mM dNTPs y 0.4µL U-Taq ADN polimerasa (SBS Genetech Co., Ltd., China).
Only for research and not intended for treatment of humans or animals