Cat. No.: PTHSR-250 (for 250U)
Cat. No.: PTHSR-2500 (for 2,500U)
For direct amplification of DNA only, please see PrimeTaq™ HotStart Direct PCR DNA Polymerase.
High DNA template dependent polymerase activity and RNA template dependent reverse transcriptase activity.
5 '- 3' exonuclease activity for TaqMan probe cleavage.
For RT-PCR, the RNA template should be no longer than 280 bp. The recommended length range is 70-150 bp.
The polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 92°C for 5 min.
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid insoluble substance within 30 min at 68°C.
Only for research and not intended for treatment of humans or animals