Cat. No.: PTPRM-1 (for 1ml)
Cat. No.: PTPRM-25 (for 25ml)
PrimeTaq™ Probe One-Step RT-qPCR MasterMix is a premix composed of optimized reaction buffer, dNTPs (0.2mM each), Mg2+ (5mM), 10% glycerol, 0.1U/μl PrimeTaq™ HS DP DNA/RNA Polymerase.
High DNA template dependent polymerase activity and RNA template dependent reverse transcriptase activity.
5 '- 3' exonuclease activity for TaqMan probe cleavage.
For RT-PCR, the RNA template should be no longer than 280 bp. The recommended length range is 70-150 bp.
The polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 92°C for 5 min.