thumbnail image

for Superior Biology Services

  • Home
  • …  
    • Home
  • 0

for Superior Biology Services

  • Home
  • …  
    • Home
  • 0
  • Home
  • Products
    • All Products
    • COVID-19
    • Custom Services
    • Catalog Products
      • PCR-Related​
      • RNA-Related​
      • Nucleic Acid Purification​
      • Nucleic Acid Stain
      • Nuclease
      • Nucleic Acid Test
      • Biochemicals​
      • DNA Markers
      • Genetic Manipulation
      • Lab Supplies​
  • About Us
    • About SBS
    • Achievements
    • Collaboration
    • Legal Statement
    • Contact
    • Old Website
  • Search
    Go Back
    PrimeTaq™ Probe qPCR MasterMix

    PrimeTaq™ Probe qPCR MasterMix

    230.00 - 1,000.00
    PrimeTaq™ Probe qPCR MasterMix provides rapid real-time quantification of DNA targets. The MasterMix contains optimized components that allow PCR amplification to take place. PrimeTaq™ Probe qPCR MasterMix is intended for molecular biology applications.
    Select
    Quantity
    Coming soon
    Coming soon
    More Details

    Cat. No.: PTPM-5 (for 5ml)

    Cat. No.: PTPM-25 (for 25ml)

     

    Detection Principle

    Probe-based qPCR uses fluorescent and quencher-labeled DNA probes to target the sequence which will be amplified by PCR. Normally, quenching groups on the probe result in quenching of fluorescent groups due to the fluorescence resonance energy transfer (FRET) in space. When the target sequence is amplified by PCR reaction, both primers and probes are annealed to the target gene. With the extension of primers, the 5' to 3' exonuclease activity of Taq enzyme will cause the probe bound to the target sequence to be degraded gradually from the 5' end. After the fluorescent group and quenching group of the probe are cleaved by Taq enzyme, the quenching group disappears, and the fluorescent group can be normally excited by the excitation light to produce fluorescence. After each PCR cycle, more fluorescent groups are released, and the fluorescence intensity is proportional to the number of newly synthesized target fragments, thus quantitative detection can be achieved. Probes are usually a fragment of linear DNA specific to the target sequence, labeled with fluorescent groups such as FAM or HEX at the 5' end and fluorescent quenching groups such as BHQ1, TAMRA or MGB at the 3' end.

     

    ROX Normalization

    By default, the MasterMix won’t contain ROX. But we can also provide Low ROX and High ROX version if needed, which will be widely compatible with fluorescent quantitative PCR instrument without ROX and requiring Low ROX or High ROX as passive reference dye.

     

    Instruction: Protocol

     

    Related: SARS-CoV-2 RT-qPCR Primers & Probes, PrimeTaq™ Probe One-Step RT-qPCR Kit

     

    Only for research and not intended for treatment of humans or animals

     

     

     

    SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

     
    •  

       

       

      Let's Create the Future of Life Science

      Explore Our Scientific Research Solution for the Study of COVID-19

      Explore Now
    • Types of Products

      We provide optimized solutions for life science research

      Custom Oligos Synthesis

      DNA synthesis is a technology that links deoxynucleic acids (adenine, thymine, cytosine and guanine) together to form DNA. As the cornerstone of modern molecular biology, DNA synthesis plays a pivotal role in the field of synthetic biology. In addition to standard oligos synthesis, we also provide scientific research services such as long oligos synthesis, phosphorothioate oligos (S-Oligo) synthesis, modified oligos synthesis, fluorescent oligos synthesis, and real-time quantitative PCR probes to meet your different needs.

      Custom Peptides Synthesis

      Peptides are synthesized by condensation reaction of carboxyl group of one amino acid with amino group of another amino acid and are widely used in various fields such as antibody preparation, drug development and polypeptide vaccine development. Each of our polypeptides is accompanied by reliable HPLC and mass spectrometry data, detailed synthesis reports are provided, and the products are sent in a lyophilized state. Experienced staff can assist users in designing peptide chains and make appropriate recommendations for different needs of users, such as antibodies, special markers, large-scale synthesis, etc. In addition, we also offer Custom PNA Synthesis with high quality.

      Custom Peptide Library

      A peptide library is a new technique for studying structure-function relationship for a protein. A peptide library contains a great number of peptides that have a systematic combination of amino acids. The peptide library provides a powerful tool for drug design, protein-protein interactions, and other biochemical as well as pharmaceutical applications. It also has wide applications in drug screening, target validation, epitope mapping, and vaccine development.

      Gene Synthesis​

      Gene synthesis is a technology that synthesizes genes by artificial methods, which is one of the means of gene acquisition. Compared with the acquisition of genes from existing organisms, gene synthesis does not need templates and is therefore not limited by the source of genes. We use unique gene synthesis design software, which includes a full set of tools to design ideal structural units, thus enabling rapid and efficient gene construction and synthesis in a single reaction. Please do not be limited by restriction sites and polylinkers, we will synthesize the various gene sequences you need.

      PCR-Related​

      Polymerase chain reaction (PCR) is a widely used method in molecular biology, which can rapidly replicate millions to billions of specific DNA samples, enabling scientists to extract only a small amount of DNA samples for detailed research. We provide a variety of DNA polymerases (Taq, Bst, f-Pfu, sPfu) and corresponding PCR premixes, covering a wide range of scenarios such as high fidelity, high specificity, and rapid amplification. Scarlet™ series kits provide a perfect solution for blood direct PCR, and PrimeSNP™ Genotyping Kit uses Competitive Allele Specific PCR method for genotyping of purified DNA samples. High quality dNTPs (set, mix) are also supplied.

      RNA-Related​

      Ribonucleic acid (RNA), as a key material for genetic information transmission and cell regulation, has been extensively studied in molecular biology. Like DNA, RNA is assembled in the form of nucleotide chains; but unlike DNA, RNA exists in nature in the form of single-stranded folds rather than paired double strands. We provide RNasin (RNase inhibitor), M-MLV reverse transcriptase and Carrier RNA to provide a complete raw material solution for RNA research. miAnalysis™ series are designed for microRNA quantitative research. And VirusMag™ series are designed for the isolation of viral RNA/DNA or bacterial DNA.

      Nucleic Acid Purification​

      Nucleic acid purification is an important component of molecular biology and has a wide range of applications in medicine and biological sciences. Our nucleic acid purification kit uses first-class silica gel column technology (SiMax™ Spin Column, SiMax™ Genomic DNA Extraction, SiMax™ PCR Products/Agarose Gel Purification, SiMax™ Plasmid DNA Miniprep) and magnetic beads technology (VirusMag™ Magnetic Beads, VSep™ Magnetic Separators, VirusMag™ One-Step DNA/RNA Isolation Kit, VirusMag™ DNA/RNA Isolation Kit), which can purify DNA from various sources quickly and reliably. The purity of DNA purified by our kit is very high and it is suitable for many downstream applications such as sequence determination, cloning and cleavage.

      DNA Markers

      DNA markers are used to determine the approximate size of molecules on the gel during electrophoresis, based on the principle that the molecular weight is inversely proportional to the mobility through the gel matrix. We provide abundant DNA molecular weight standards that can be used for various fragment lengths. Our fragments of DNA markers have been purified separately by proprietary technology, so their quality is superior to industry standards.

      Genetic Manipulation

      Gene manipulation is a process that uses biotechnology to manipulate genes directly to generate new DNA, and has been widely used in research, medicine, industrial biotechnology and agriculture. Our Premium™ Master Assembly Mix, Muta-direct™ Site-Directed Mutagenesis Kit and Topo Cloning kits (pBM23, pBM16A, pBM16K) provide efficient solutions for these types of demands. We also provide high quality chemically modified Synthetic sgRNAs and Cas nucleases to provide a more reliable solution for CRISPR gene editing.

      Nucleic Acid Stain

      GoodView™ is a safer nucleic acid stain, an alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 268 nm and another at 294 nm. In addition, it has one visible excitation at 491 nm. The Fluorescence emission of GoodView bound to DNA is centered at 530 nm. Our GoodView™ Nucleic Acid Stain is also included on New Products, Science Magazine, January 11, 2019.

      Biochemicals​

      Biochemistry, as its name implies, is a discipline that studies chemical processes in organisms, often referred to as biochemistry. It is mainly used to study the structure and function of various intracellular components, such as proteins, sugars, lipids, nucleic acids and other biomacromolecules. We provide variety types of high quality biochemical reagents to meet these demands, including Proteinase K, Mutant Proteinase K, Besta™ LE Agarose, IPTG, X-Gal, DNase I (RNase Free), Enterokinase, SUMO Protease, TEV Protease, PreScission Protease (PSP), Bovine Serum Albumin, T4 DNA Ligase, etc.

      Nuclease

      A nuclease is an enzyme capable of cleaving the phosphodiester bonds between nucleotides of nucleic acids. Nucleases variously effect single and double stranded breaks in their target molecules. Here we offer various high quality nucleases, including RNase A, RNase H, Thermostable RNase H, Benzonase Nuclease, Uracil N-Glycosylase, T7 Endonuclease I, T5 Exonuclease, Shrimp Alkaline Phosphatase, Terminal Transferase, etc.

      Nucleic Acid Test

      A nucleic acid test (NAT) is a technique used to detect a particular nucleic acid sequence and thus usually to detect and identify a particular species or subspecies of organism, often a virus or bacteria that acts as a pathogen in blood, tissue, urine, etc. Based on our leading fluorescent quantitative PCR technique and isothermal amplification technology, we have developed solutions for various pathogens.

      Lab Supplies​

      We provide various high quality common lab supplies, including ELISA Plates, Cell Culture Dishes, Cell Culture Plates, Microcentrifuge Tube, Centrifuge Bottles, Centrifuge Tubes, PCR Plates, PCR Tubes, Pipette Tips, Serological Pipettes, 96-well Deep-Well Plate, Conical Cryovials, SiMax™ Spin Column, with high cost-effective ratio to make your experiments smoother!

    • Why Choose Us?

    • Reliable Global Supply Chain

      With reliable GSC, we are recognized as one of the leading APAC biotech B2B suppliers.

      Learn More

      Trusted by Top Researchers

      Our high quality products are trusted by researchers and scientists in top universities and institutes.

      Learn More

      Powerful Technical Support

      Our support personnel are all experts with average more than 10 years of working experience.

      Learn More
    • SBS Genetech is a long-term sponsor of

      Cold Spring Harbor Laboratory

      Learn More
    • Contact

      For more information, please fill out the provided form or contact us directly by E-mail

      tech@sbsbio.com
      Submit

    SBS Genetech © Copyright 2020

      Home
      Shop
      Papers
    All Posts
    ×
    Cookie Use
    We use cookies to ensure a smooth browsing experience. By continuing we assume you accept the use of cookies.
    Learn More