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Cat. No.: NTBB-150 (for 150U)

Recognition Site

CCTCAGC
5'...C C↓T C A G C...3'
3'...G G A G T C G...5'

Description

Nt.BbvCI is a nicking endonuclease that specifically cleaves only one strand of double-stranded DNA (dsDNA) substrates, generating a single-strand nick without completely severing the double helix. This enzyme is widely employed in isothermal nucleic acid amplification techniques such as Strand Displacement Amplification (SDA) and Rolling Circle Amplification (RCA). The nick introduced by Nt.BbvCI activates the strand displacement activity of associated polymerases, initiating a continuous cycle of nicking, strand displacement, and DNA extension. This iterative process ultimately leads to exponential amplification of the target nucleic acid sequence.​

Recommended Reaction Conditions

• 1× RapidCleave™ Buffer;
• Incubation at 37°C;
• Refer to the "DNA Rapid Cleavage Protocol" for reaction setup.

Inactivation Conditions

Incubate at 80°C for 20 minutes.

Methylation Sensitivity

• Cutting may be hindered for DNA methylated by EcoBI.