RapidCleave™ Nb.BbvCI (300U)
$120.00
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Cat. No.: NBBV-300 (for 300U)
Recognition Site
CCTCAGC
5'...C C T C A G C...3'
3'...G G A G T↑C G...5'
Description
Nb.BbvCI is a nicking endonuclease that cleaves only one strand of a double-stranded DNA (dsDNA) substrate, generating a single-strand nick without disrupting the overall duplex structure. It is commonly used in nucleic acid isothermal amplification techniques such as SDA (Strand Displacement Amplification) and RCA (Rolling Circle Amplification). In these applications, the nick introduced by Nb.BbvCI initiates a polymerase-driven strand displacement reaction. Through repeated cycles of nicking, displacement, and extension, exponential amplification of nucleic acids is achieved.
Recommended Reaction Conditions
- 1× RapidCleave™ Buffer;
- Incubation at 37°C;
- Refer to the "DNA Rapid Cleavage Protocol" for reaction setup.
Inactivation Conditions
Incubate at 80°C for 20 minutes.
Methylation Sensitivity
- Cutting may be hindered for DNA methylated by EcoBI.
Nicking Endonucleases:
