CM HS-G3Taq DNA Polymerase (Glycerol Free)
$80.00 - $240.00
$300.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: CMHSG3-250 (for 250U)
Cat. No.: CMHSG3-1k (for 1000U)
Description
G3Taq DNA Polymerase is a third-generation Taq DNA polymerase featuring 5’→3’ exonuclease activity but lacking 3’→5’ exonuclease (proofreading) activity. It exhibits exceptional tolerance to impurities such as ethanol, guanidine salts, and heparin, enabling it to deliver reliable results even with low-purity DNA templates.
CM HS-G3Taq DNA Polymerase is a chemically modified HotStart version of the enzyme. It remains completely inactive below 50°C and regains full activity only after heating at 95°C for 5 minutes. This feature effectively suppresses nonspecific amplification and significantly enhances PCR specificity and sensitivity.
The enzyme is supplied with a proprietary reaction buffer, optimized to support robust amplification across a broad range of conditions, yielding higher sensitivity and stronger signal intensity.
Features
- Amplification Performance: Capable of amplifying DNA fragments up to 4 kb from λDNA and up to 2 kb from human genomic DNA.
- High Specificity: Remains completely inactive below 50°C and requires heating at 95°C for 5 minutes to fully restore enzymatic activity.
Unit Definition
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
Storage
Stable for up to 3 years at -20°C.
Performance Comparison of HotStart G3Taq Polymerases
HotStart Mechanism | CM HS-G3Taq Chemical modification | AP HS-G3Taq Aptamer-based blocking |
Inactivation Efficiency | 100% inactive below 50°C | ~90% inactive below 37°C |
Enzyme Activation | 95°C for 5 minutes | Above 50°C |
Average Extension Rate | 1 kb/min | 2 kb/min |
Amplification Capability | Up to 2 kb (human gDNA) | Up to 8 kb (human gDNA) |
Recommended Applications | Multiplex and short-fragment amplification with high specificity | Long-fragment amplification with high specificity |
Related:
- CM HS-G3Taq DNA Polymerase (Glycerol Free)
- 5× CM HS-G3Taq SYBR Green qPCR Mix
- 5× CM HS-G3Taq Probe Mix (Lyo-Ready)
- 5× CM HS-G3mTTx qRT-PCR Mix (Lyo-Ready)
- 3M Spherical Lyophilization Excipient
- AP HS-G3Taq DNA Polymerase (Glycerol Free)
- 5× DualHS G3mTTx qPCR Mix (Lyo-Ready)
SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data
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