5× DualHS G3mTTx qPCR Mix (Lyo-Ready)
$300.00 - $1,200.00
$1,500.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: DHSG3-100 (for 100Tx20µl)
Cat. No.: DHSG3-500 (for 500Tx20µl)
Description
5× DualHS G3mTTx qPCR Mix is a specialized 5× master mix for TaqMan-based Real-Time PCR, featuring dual aptamer-based HotStart enzymes—G3Taq DNA Polymerase and Extreme ThermoStable Reverse Transcriptase (ET RTase)—along with dNTPs, Mg²⁺ activator, Mg²⁺ ions, and stabilizers. The mix enables bias-free amplification of both DNA and RNA under Mg²⁺-dependent conditions (no Mn²⁺ required) and supports ultrasensitive detection of nucleic acids.
Due to the extreme heat resistance of ET RTase (retaining activity after 95°C treatment), the system allows direct detection of crude samples—thermal lysis efficiently releases nucleic acids from both DNA and RNA viruses, eliminating the need for nucleic acid purification prior to amplification.
Features
- Dual Aptamer-Based HotStart Enzymes: Both GSTaq and ET RTase are aptamer-blocked, maintaining >90% inactivity below 30°C and fully activating above 50°C. For experiments requiring enhanced HotStart stringency, a separate Blocker component can be added to sequester Mg²⁺ through insoluble complex formation. After heating at 95°C for 2 minutes, the Activator hydrolyzes the Blocker, releasing Mg²⁺ to initiate the reaction, achieving dual-layered HotStart control.
- Thermostable RT Performance: ET RTase maintains full reverse transcriptase activity after 15 minutes at 95°C. Its active center has been engineered to switch metal ion dependence from Mn²⁺ to Mg²⁺, enabling true one-step RT-PCR.
- Amplification Range: When using RNA templates, the system supports RT-PCR amplicons up to 500 bp, with optimal performance in the 70–150 bp range.
- Glycerol-Free and Lyophilization-Ready: The mix is free of glycerol and fully compatible with the proprietary 3M Spherical Lyophilization Excipient, simplifying the preparation of lyophilized PCR bead formulations. This integrated design streamlines research, development, and production, offering flexibility while reducing the challenges of reagent optimization and immobilization.
Storage
Stable for up to 3 years at -20°C.
Performance Comparison of HotStart G3Taq Polymerases
HotStart Mechanism | CM HS-G3Taq Chemical modification | AP HS-G3Taq Aptamer-based blocking |
Inactivation Efficiency | 100% inactive below 50°C | ~90% inactive below 37°C |
Enzyme Activation | 95°C for 5 minutes | Above 50°C |
Average Extension Rate | 1 kb/min | 2 kb/min |
Amplification Capability | Up to 2 kb (human gDNA) | Up to 8 kb (human gDNA) |
Recommended Applications | Multiplex and short-fragment amplification with high specificity | Long-fragment amplification with high specificity |
Related:
- CM HS-G3Taq DNA Polymerase (Glycerol Free)
- 5× CM HS-G3Taq SYBR Green qPCR Mix
- 5× CM HS-G3Taq Probe Mix (Lyo-Ready)
- 5× CM HS-G3mTTx qRT-PCR Mix (Lyo-Ready)
- 3M Spherical Lyophilization Excipient
- AP HS-G3Taq DNA Polymerase (Glycerol Free)
- 5× DualHS G3mTTx qPCR Mix (Lyo-Ready)
SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data
Only for research and not intended for treatment of humans or animals
Journals Using SBS Genetech Products Universities Using SBS Genetech Products
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory
