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Beijing SBS Genetech Co.,Ltd.
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Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

for Superior Biology Services

  • Home
  • Products 
    • All Products
    • COVID-19
    • Custom Services
    • Catalog Products
    • Isothermal Portfolio
    • Innovative Systems
  • About Us 
    • About SBS
    • Achievements-Journals
    • Achievements-Universities
    • Collaboration
    • Legal Statement
    • Contact
    • Ecosystem
  • Synthetic Biology
  • …  
    • Home
    • Products 
      • All Products
      • COVID-19
      • Custom Services
      • Catalog Products
      • Isothermal Portfolio
      • Innovative Systems
    • About Us 
      • About SBS
      • Achievements-Journals
      • Achievements-Universities
      • Collaboration
      • Legal Statement
      • Contact
      • Ecosystem
    • Synthetic Biology
  • 0
  • Login
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    • 简体中文
Beijing SBS Genetech Co.,Ltd.
  •  

    Isothermal Amplification

    At SBS Genetech, we are at the forefront of offering solutions for isothermal amplification

    based on our world-class platform

  • It's time for Isothermal Amplification

    Nucleic acid testing technology has developed rapidly in recent years, especially in the field of molecular diagnosis. At present, the mainstream of nucleic acid testing techniques in the market is based on quantitative PCR technology (qPCR), which features strong specificity and high sensitivity. This technology can quantitatively detect pathogens and achieve high-throughput detection.

    However, the amplification process of qPCR requires thermal cycling instruments. Due to their large volume, high price, and long reaction time, the application of thermal cycling instruments in grass-roots units and on-site detection is very limited.

    In the past 20 years, scientists have been trying to overcome the above shortcomings through isothermal amplification, including Rolling Circle Amplification (RCA), Loop-mediated isothermal amplification (LAMP), Recombinase Polymerase Amplification (RPA), Nucleic Acid Sequence-Based Amplification (NASBA), Strand Displacement Amplification (SDA), Helicase-dependent Amplification (HDA), Transcription-mediated Amplification (TMA) and other isothermal amplification technologies. With these developments, isothermal amplification is now considered by many researchers and scientists as a detection method that may be comparable with qPCR technology.

    Learn More
  • Bst DNA/RNA Polymerase

    At the core of our world-class platform

    Our Bst DNA/RNA Polymerase is a mixture of Bst polymerase and extremely thermostable reverse transcriptase (65°C tolerant), which is suitable for isothermal amplification reaction of RNA. It can detect low-sensitivity RNA molecules. This enzyme is recommended in isothermal amplification experiments using RNA as a template. In addition, Bst DNA/RNA Polymerase can also perform isothermal amplification of DNA templates.

    Learn More
  • Higher Specificity

    By unique negative control technique

    Better Sensitivity

    By optimized reaction buffer

    Lower False Positive Rate

    By strong recognition ability to dUTP

    Lyophilized Version Available

    By advanced freeze-drying technology

  • common Bst DNA Polymerase

    our Bst DNA/RNA Polymerase

  • Comparison

    SBS Genetech's isothermal amplification technology has excellent performance

    Compared with PCR, SBS Genetech's Isothermal Amplification Technology has the advantages of Shorter Reaction Time and Easier Program.

     

    While Compared with conventional isothermal amplification, SBS Genetech's Isothermal Amplification Technology has the advantages of Higher Specificity, Better Sensitivity, Lower False Positive Rate, and Clearer Observable Results.

  • PrimeIAmp™ Series

    Advanced solutions based on our Bst DNA/RNA Polymerase

    Anti-contamination Solution

    PrimeIAmp™ Basic Isothermal Amplification MasterMix (with UDG), an upgraded version of PrimeIAmp™ Basic Isothermal Amplification MasterMix, is a two-component reagent. The buffer mix contains reaction buffer, Mg2+, dU/A/C/GTP (excluding dTTP), and lyophilization excipient. The enzyme mix contains Bst DNA/RNA polymerase and heat-labile UDG. Thanks to the recognition of the dUTP substrate by Bst DNA/RNA Polymerase, the reaction substrate does not contain dTTP, so the amplified products are dUTP products, which can achieve anti-contamination in combination with heat-labile UDG. In the actual test, the amplification of contaminates was completely inhibited under the condition of containing 105 copies of contaminates.

    Learn More

    Room Temperature Transportation Solution

    PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads contain reaction buffer, Mg2+, dNTP, Bst DNA/RNA polymerase, and so on in the lyophilized form. Only primers and templates are needed to be added for the isothermal amplification. Like the basic version of PrimeIAmp™ Lyophilized Isothermal Amplification Microbeads series, this version does not contain any dyes and can be flexibly used for various isothermal amplification applications (e.g. OG Colorimetric Tube). PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads can be stored at room temperature (25°C) for more than 6 months.

    Learn More

    Colorimetric Visualization Solution

    PrimeIAmp™ HNB Lyophilized Isothermal Amplification Microbeads contain reaction buffer, HNB, Mg2+, dNTP, Bst DNA/RNA polymerase, and so on in the lyophilized form. Only primers and templates are needed to be added for the isothermal amplification. After amplification, the positive samples are sky blue, while the negative samples are violet. The results can be directly observed by naked eyes without any other auxiliary equipment. PrimeIAmp™ HNB Lyophilized Isothermal Amplification Microbeads are so far one of the most robust systems for colorimetric isothermal amplification as it has a very good tolerance to pH and buffer salt.

    Learn More
  • BodyIAmp™ Series

    The core technology of the BodyIAmp™ is its unique body temperature amplification technology, which is based on a set of combined enzyme preparations that can efficiently amplify trace nucleic acid templates.
     
    Through enzyme engineering, the specific tool enzymes originating from bacteria, viruses, and phages are modified and mutated, and their functions are screened. Different nucleic acid amplification reaction systems are optimized and combined to obtain a core recombinant isothermal amplification system.
     
    Using the modified recombinases combined with primers to form a protein DNA complex, the homologous sequence is found in double-stranded DNA and DNA synthesis will be initiated. The target gene on the template will be exponentially amplified. Due to the high specificity of the enzyme, the generation of mismatches is reduced, thereby reducing the probability of test errors.

    Rapid Amplification

    In most cases, trace nucleic acid samples can be amplified to detectable levels within 20 minutes.

    High Stability

    The product is freeze-dried and can be transported at room temperature. It can be stored at -20°C for more than one year.

    High Sensitivity

    The detection limit can reach 10-100 copies/reaction.

    Easy operation

    The main components of the product are prefabricated into microspheres by an advanced freeze-drying process, without the need for professional equipment and training. The whole process is easy to operate.

  •  

    Ready for this new experience?

     

  • Isothermal Amplification Products

  • More Information

  • Published Papers

    Representative Publications Using SBS Genetech Isothermal Amplification Products

     

    Xue, T. , Ma, Z. , Liu, F. , Du, W. , & An, C. . (2020). Pneumocystis jirovecii colonization and its association with pulmonary diseases: a multicenter study based on a modified loop-mediated isothermal amplification assay. BMC Pulmonary Medicine, 20(1).

     

    Zhao, K. , Hu, R. , Ni, J. , Liang, J. , & Li, C. . (2020). Establishment of a porcine parvovirus (PPV) LAMP visual rapid detection method. Journal of Virological Methods, 284, 113924.

     

    Papadakis, G. , Pantazis, A. K. , Fikas, N. , Chatziioannidou, S. , Tsiakalou, V. , & Michaelidou, K. , et al. (2022). Portable real-time colorimetric LAMP-device for rapid quantitative detection of nucleic acids in crude samples. Scientific Reports, 12(1), 1-15.

     

    Wu, R. , Liu, X. , Guo, B. , Chen, F. , & Wang, X. . (2014). Development of double loop-mediated isothermal amplification to detect listeria monocytogenes in food. Current Microbiology, 69(6), 839-845.

     

    Ji, J. , Du, L. Q. , Xie, Q. M. , Cao, Y. C. , & Bee, Y. Z. . (2009). Rapid diagnosis of duck plagues virus infection by loop-mediated isothermal amplification. Research in Veterinary Science, 87(1), 53-58.

     

    Zhang, S. , Xu, X. , Wu, Q. , & Zhang, J. . (2013). Rapid and sensitive detection of pseudomonas aeruginosain bottled water by loop-mediated isothermal amplification. European Food Research & Technology, 236(1), 209-215.

     

    Xu, X. , Zhang, S. , Wu, Q. , Zhang, J. , Li, F. , & Cheng, J. . (2014). Development and Application of a Loop-Mediated Isothermal Amplification (LAMP) Method for Rapid and Sensitive Detection of Enterococcus faecalis in Drinking Water. Journal of Food Safety, 34(2), 103–110.

     

    Zhang, F. , Shi, Y. , Jiang, K. , Song, W. , Ma, C. , & Xu, Z. , et al. (2014). Rapid detection and quantification of Prorocentrum minimum by loop-mediated isothermal amplification and real-time fluorescence quantitative PCR. Journal of Applied Phycology, 26(3), 1379-1388.

  • Cold Spring Harbor Laboratory - SBS Genetech

    SBS Genetech is a long-term sponsor of

    Cold Spring Harbor Laboratory

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  • Contact

    For more information, please fill out the provided form or contact us directly by E-mail

    tech@sbsbio.com
    Submit

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