Nucleic acid testing technology has developed rapidly in recent years, especially in the field of molecular diagnosis. At present, the mainstream of nucleic acid testing techniques in the market is based on quantitative PCR technology (qPCR), which features strong specificity and high sensitivity. This technology can quantitatively detect pathogens and achieve high-throughput detection.
However, the amplification process of qPCR requires thermal cycling instruments. Due to their large volume, high price and long reaction time, the application of thermal cycling instruments in grass-roots units and on-site detection are very limited.
Compared with other nucleic acid testing techniques, isothermal amplification has the advantages of high sensitivity and rapidity of reaction, and does not need special equipment. Therefore, isothermal amplification has been considered by many researchers and scientists as a detection method which may be comparable with qPCR technology.
Bst DNA/RNA Polymerase
At the core of our world-class platform
Our Bst DNA/RNA Polymerase is a mixture of Bst polymerase and extremely thermostable reverse transcriptase (65°C tolerant), which is suitable for isothermal amplification reaction of RNA. It can detect low-sensitivity RNA molecules. This enzyme is recommended in isothermal amplification experiments using RNA as a template. In addition, Bst DNA/RNA Polymerase can also perform isothermal amplification of DNA templates.
SBS Genetech's isothermal amplification technology has excellent performance
Compared with PCR, SBS Genetech's Isothermal Amplification Technology has the advantages of Shorter Reaction Time and Easier Program.
While Compared with conventional isothermal amplification, SBS Genetech's Isothermal Amplification Technology has the advantages of Higher Specificity, Better Sensitivity, Lower False Positive Rate, and Clearer Observable Results.