What is Loop-mediated Isothermal Amplification (LAMP)?
Loop-mediated Isothermal Amplification (LAMP) is a rapid, highly sensitive, and highly specific nucleic acid amplification technology that enables DNA or RNA detection under a constant temperature. Unlike conventional Polymerase Chain Reaction (PCR), which relies on repeated thermal cycling, LAMP continuously amplifies target nucleic acids at a single temperature—typically between 60°C and 65°C—using a strand-displacing DNA polymerase and a specially designed set of primers.
Because the reaction does not require a thermal cycler, LAMP greatly simplifies molecular testing while maintaining excellent analytical performance. The technology has become one of the most widely adopted isothermal amplification methods for applications ranging from life science research to molecular diagnostics, food safety, veterinary testing, and point-of-care testing (POCT).
Today, LAMP serves as a core technology for rapid molecular detection, especially in scenarios where speed, portability, and operational simplicity are essential.
What Makes LAMP Different?
The fundamental difference between LAMP and conventional PCR lies in the amplification strategy.
PCR requires repeated cycles of DNA denaturation, primer annealing, and polymerase extension, making a precision thermal cycler indispensable. In contrast, LAMP performs continuous DNA synthesis at a constant temperature through the coordinated action of multiple primers and a strand-displacing DNA polymerase.
This isothermal mechanism not only simplifies instrument requirements but also significantly shortens assay time. In many applications, amplification can be completed within 15–30 minutes, allowing rapid molecular testing without sacrificing sensitivity or specificity.
For laboratories developing portable or decentralized diagnostic solutions, the ability to perform amplification using only a simple heating device represents one of LAMP's greatest advantages.
How Does LAMP Work?
LAMP utilizes four to six primers that recognize six to eight distinct regions on the target nucleic acid sequence. This unique primer design generates loop structures during amplification, enabling continuous self-primed DNA synthesis throughout the reaction.
Instead of repeatedly separating DNA strands through heating, LAMP relies on a strand-displacing DNA polymerase, such as Bst DNA polymerase, to synthesize new DNA while simultaneously displacing existing strands. This process rapidly produces large quantities of amplification products with exceptional efficiency.
For RNA targets, reverse transcriptase can be incorporated into the same reaction, allowing Reverse Transcription LAMP (RT-LAMP) to perform reverse transcription and DNA amplification in a single tube, reducing workflow complexity and minimizing contamination risk.
Powering Next-Generation LAMP with Advanced Bst Polymerases
The evolution of LAMP has been closely linked to continuous advances in strand-displacing DNA polymerases, particularly Bst DNA polymerases, which serve as the foundation of efficient isothermal amplification. As LAMP applications have expanded from basic DNA detection to rapid RNA detection, RT-LAMP, multiplex assays, lyophilized molecular tests, and point-of-care diagnostics, next-generation polymerases have been engineered to deliver faster amplification kinetics, enhanced substrate compatibility, improved inhibitor tolerance, and greater reaction robustness.
At SBS Genetech, enzyme engineering is at the core of our LAMP technology platform. Our latest generation of advanced Bst polymerases integrates high amplification efficiency, Hot Start control, DNA/RNA co-amplification capability, and complete dUTP compatibility for carryover contamination prevention. These innovations provide researchers and diagnostic developers with a reliable foundation for developing fast, sensitive, and robust next-generation LAMP assays.
The Next Step in Bst Polymerase Evolution
As part of SBS Genetech’s LAMP enzyme engineering portfolio, Bst dU-P DNA/RNA Polymerase represents the culmination of multiple generations of optimization, combining hot-start control, DNA/RNA amplification capability, 65–70°C robustness, inhibitor tolerance, and 100% dUTP compatibility into a single next-generation platform.
Lyophilized LAMP Assay System Platform
Lyophilized LAMP Assay Systems are fully pre-mixed, freeze-dried molecular detection solutions designed to simplify nucleic acid amplification workflows while significantly improving reagent stability and transportability. By integrating all essential reaction components into a single lyophilized format, the system enables rapid deployment of DNA and RNA detection assays without the need for cold-chain storage or complex reagent preparation.
Built on SBS Genetech engineered LAMP enzyme technologies, including proprietary polymerase systems, the platform supports robust isothermal amplification across a wide range of diagnostic and research applications.
Available product formats include PrimeIAmp™ HotStart Bst dU-P Basic Microbeads (with UDG) for general-purpose LAMP workflows, and PrimeIAmp™ HotStart Bst dU-P Fluorescent Microbeads (with UDG) optimized for fluorescence-based real-time detection.
Loop-mediated Isothermal Amplification (LAMP) Products
Bst dU-P DNA/RNA Polymerase (glycerol-free)$280.00 - $1,856.00$2,320.00
PrimeIAmp™ HotStart Bst dU-P Fluorescent Microbeads (with UDG)$500.00 - $2,000.00$2,500.00PrimeIAmp™ HotStart Bst dU-P Basic Microbeads (with UDG)$500.00 - $2,000.00$2,500.00Bst dU‑P Fluorescent LAMP Lyophilized Mix$400.00 - $1,600.00$2,000.00Bst dU‑P Basic LAMP Lyophilized Mix$400.00 - $1,600.00$2,000.004× Bst dU‑P Fluorescent LAMP Master Mix (glycerol-free)$400.00 - $1,600.00$2,000.004× Bst dU‑P Basic LAMP Master Mix (glycerol-free)$400.00 - $1,600.00$2,000.00
Mycoplasma Pneumoniae Fluorescence LAMP Assay Kit$196.00 - $596.00$745.00HotStart Bst P DNA/RNA Polymerase (glycerol-free)$280.00 - $1,856.00$2,320.00HotStart Bst DNA/RNA Polymerase (glycerol-free)$280.00 - $1,856.00$2,320.00PrimeIAmp™ HotStart LAMP Bst P L-HNB MasterMix$400.00 - $1,600.00$2,000.00PrimeIAmp™ HotStart Bst P DNA/RNA LHNB LyoMix (70ºC reaction)$660.00 - $2,000.00$2,500.00PrimeIAmp™ HotStart Bst P DNA/RNA Basic & SYBR Green LyoMix (70ºC reaction)$660.00 - $2,000.00$2,500.00PrimeIAmp™ HotStart Bst P DNA/RNA LHNB Microbead (70ºC reaction)$740.00$925.00PrimeIAmp™ HotStart Bst P DNA/RNA Basic Microbead (70ºC reaction)$740.00$925.00PrimeIAmp™ HotStart Bst DNA/RNA LHNB LyoMix (65ºC reaction)$660.00 - $2,000.00$2,500.00PrimeIAmp™ HotStart Bst DNA/RNA Basic & SYBR Green LyoMix (65ºC reaction)$660.00 - $2,000.00$2,500.00PrimeIAmp™ HotStart Bst DNA/RNA LHNB Microbead (65ºC reaction)$740.00$925.00PrimeIAmp™ HotStart Bst DNA/RNA Basic Microbead (65ºC reaction)$740.00$925.00Trusted by Researchers Worldwide
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