It's time for Isothermal Amplification
Nucleic acid testing technology has developed rapidly in recent years, especially in the field of molecular diagnosis. At present, the mainstream of nucleic acid testing techniques in the market is based on quantitative PCR technology (qPCR), which features strong specificity and high sensitivity. This technology can quantitatively detect pathogens and achieve high-throughput detection.
However, the amplification process of qPCR requires thermal cycling instruments. Due to their large volume, high price, and long reaction time, the application of thermal cycling instruments in grass-roots units and on-site detection is very limited.
In the past 20 years, scientists have been trying to overcome the above shortcomings through isothermal amplification, including Rolling Circle Amplification (RCA), Loop-mediated isothermal amplification (LAMP), Recombinase Polymerase Amplification (RPA), Nucleic Acid Sequence-Based Amplification (NASBA), Strand Displacement Amplification (SDA), Helicase-dependent Amplification (HDA), Transcription-mediated Amplification (TMA) and other isothermal amplification technologies. With these developments, isothermal amplification is now considered by many researchers and scientists as a detection method that may be comparable with qPCR technology.
Bst DNA/RNA Polymerase
At the core of our world-class platform
Our Bst DNA/RNA Polymerase is a mixture of Bst polymerase and extremely thermostable reverse transcriptase (65°C tolerant), which is suitable for isothermal amplification reaction of RNA. It can detect low-sensitivity RNA molecules. This enzyme is recommended in isothermal amplification experiments using RNA as a template. In addition, Bst DNA/RNA Polymerase can also perform isothermal amplification of DNA templates.
common Bst DNA Polymerase
our Bst DNA/RNA Polymerase
Comparison
SBS Genetech's isothermal amplification technology has excellent performance
Compared with PCR, SBS Genetech's Isothermal Amplification Technology has the advantages of Shorter Reaction Time and Easier Program.
While Compared with conventional isothermal amplification, SBS Genetech's Isothermal Amplification Technology has the advantages of Higher Specificity, Better Sensitivity, Lower False Positive Rate, and Clearer Observable Results.
PrimeIAmp™ Series
Advanced solutions based on our Bst DNA/RNA Polymerase
Anti-contamination Solution
PrimeIAmp™ Basic Isothermal Amplification MasterMix (with UDG), an upgraded version of PrimeIAmp™ Basic Isothermal Amplification MasterMix, is a two-component reagent. The buffer mix contains reaction buffer, Mg2+, dU/A/C/GTP (excluding dTTP), and lyophilization excipient. The enzyme mix contains Bst DNA/RNA polymerase and heat-labile UDG. Thanks to the recognition of the dUTP substrate by Bst DNA/RNA Polymerase, the reaction substrate does not contain dTTP, so the amplified products are dUTP products, which can achieve anti-contamination in combination with heat-labile UDG. In the actual test, the amplification of contaminates was completely inhibited under the condition of containing 105 copies of contaminates.
Room Temperature Transportation Solution
PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads contain reaction buffer, Mg2+, dNTP, Bst DNA/RNA polymerase, and so on in the lyophilized form. Only primers and templates are needed to be added for the isothermal amplification. Like the basic version of PrimeIAmp™ Lyophilized Isothermal Amplification Microbeads series, this version does not contain any dyes and can be flexibly used for various isothermal amplification applications (e.g. OG Colorimetric Tube). PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads can be stored at room temperature (25°C) for more than 6 months.
Colorimetric Visualization Solution
PrimeIAmp™ HNB Lyophilized Isothermal Amplification Microbeads contain reaction buffer, HNB, Mg2+, dNTP, Bst DNA/RNA polymerase, and so on in the lyophilized form. Only primers and templates are needed to be added for the isothermal amplification. After amplification, the positive samples are sky blue, while the negative samples are violet. The results can be directly observed by naked eyes without any other auxiliary equipment. PrimeIAmp™ HNB Lyophilized Isothermal Amplification Microbeads are so far one of the most robust systems for colorimetric isothermal amplification as it has a very good tolerance to pH and buffer salt.
SBS Genetech is a long-term sponsor of
Cold Spring Harbor Laboratory
