PrimeIAmp™ Basic Isothermal Amplification MasterMix (with UDG), an upgraded version of PrimeIAmp™ Basic Isothermal Amplification MasterMix, is a two-component reagent. The buffer mix contains reaction buffer, Mg2+, dU/A/C/GTP (excluding dTTP), and lyophilization excipient. The enzyme mix contains Bst DNA/RNA Polymerase and heat-labile UDG.
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: IAMPBU-100 (for 100T)
Cat. No.: IAMPBU-2k (for 2,000T)
We also provide this MasterMix in lyophilized form, with customized primers incorporated. The minimum order quantity for 8-strip tubes is 480T, and the minimum order quantity of lyophilized microbeads is 2000T. Please contact us or email firstname.lastname@example.org for more details.
PrimeIAmp™ Basic Isothermal Amplification MasterMix (with UDG), an upgraded version of PrimeIAmp™ Basic Isothermal Amplification MasterMix, is a two-component reagent. The buffer mix contains reaction buffer, Mg2+, dU/A/C/GTP (excluding dTTP), and lyophilization excipient. The enzyme mix contains Bst DNA/RNA polymerase and heat-labile UDG. Thanks to the recognition of the dUTP substrate by Bst DNA/RNA Polymerase, the reaction substrate does not contain dTTP, so the amplified products are dUTP products, which can achieve anti-contamination in combination with heat-labile UDG. In the actual test, the amplification of contaminates was completely inhibited under the condition of containing 105 copies of contaminates.
This product is especially suitable for the isothermal amplification reaction with opening the cover (such as test strip detection) and the detection test of the sealed cavity with high contamination risk (such as quantitative PCR cavity).
The buffer mix does not contain dyes. SYBR green, Eva Green, and other dyes can be added for fluorescence detection.
The reagent is suitable for open cover detection, such as nucleic acid test strip detection.
The reagent contains lyophilization excipients, which can be directly lyophilized without optimizing the freeze-drying formula.
The minimum shelf life is 1 year at -20°C and 2 years at -80°C. For short-term use, store it at 2-8°C for 1 month. Repeated freezing and thawing 10 times will not affect the use. If there are white precipitates, dissolve the precipitates by placing in a 37°C water bath for 10 min, which will not affect the use.
Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.
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