CRISPR Gene Editing​

Our CRISPR-dCas9 Nuclese is from Streptococcus pyogenes. The PAM sequence of dCas9 is 5-NGG, which is highly abundant in the genome. dCas9 protein is a ribozyme deficient Cas9 protein, which does not have sgRNA guided DNA strand cleavage activity, but retains target DNA binding activity.

Cas9-C-NLS Nuclease is a recombinant Streptococcus pyogenes Cas9 (wt) protein. Nucleic localization signal (NLS) is added at C-terminal which can be used for genome editing by inducing site-specific DNA double stranded breaks. Cas9 protein can form ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system, which can localize to the nucleus immediately once entering the cell with the guide of the NLS.