With excellent sgRNA synthesis and purification technology, we provide high-quality synthetic sgRNAs, which can be applied in various genome engineering. Our exclusive long-chain RNA synthesis technology can support around 120nt sgRNA synthesis. Normally we would provide 2'-O-methyl 3' phosphorothioate modifications in the first and last 3 nucleotides but we can also provide other types of customized modifications.
- Our professional sgRNA synthesis and purification technology will make your experiment more likely to be successful.
- We provide high-precision mass spectrometry reports.
- We can ensure a single main peak and theoretical molecular weight consistent with the detected molecular weight.
- The purity is greater than 90%.
We recommend that RNA should be stored at -80°C in either dry powder or solution state. RNA in solution state should also be avoided from repeated freezing and thawing. Modified oligonucleotides are recommended to be stored at -20°C.
Interested in seeing published research using our gRNAs?
DDR1 promotes breast tumor growth by suppressing antitumor immunity
Oncology Reports | 27 September 2019 | DOI: https://doi.org/10.3892/or.2019.7338
Oligonucleotides with BsmB1 restriction sites for guide RNAs were synthesized by SBS Genetech Co., Ltd., then phosphorylated using T4 kinase (31). The phosphorylated oligonucleotides were cloned into LentiCRISPR v2 (Plasmid no. 52961; Addgene) and the sequences of the cloned plasmids that were extracted from numerous selected colonies were confirmed by SBS Genetech Co., Ltd.
Only for research and not intended for treatment of humans or animals
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory