In Vitro-transcribed (IVT) sgRNA is a more advanced alternative to plasmid-expressed sgRNA. The IVT sgRNA is first transcribed from a DNA template by RNA polymerase. Additional purification is then required before the gene-editing experiment.
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At SBS Genetech, we are providing in vitro-transcribed (IVT) sgRNA of high purity and kits are available if customers want to prepare the IVT sgRNA by themselves. Compared with plasmid-expressed sgRNA, IVT sgRNA is faster and easier to use. Without the plasmid construction step, the whole process takes 20 hours only (including overnight incubation). After purification, IVT sgRNA can be used directly for gene editing.
Easier preparation: Without the need for a plasmid construction step.
Faster process: The whole process takes 20 hours only (including overnight incubation).
Simpler operation: IVT sgRNA can be used directly for the gene-editing after purification.