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Description
At SBS Genetech, we can construct shRNA library and gRNA library according to customers' needs, including whole-genome gene silencing, gene knockout, gene transcription activation, and transcription inhibition. At the same time, we provide high-throughput gene-editing services by using shRNA library or gRNA library and screening services for drug target genes related to diseases by using high-throughput gene-editing technology.
Applications
Forward screening: Screen which gene mutations cause cell tolerance to different treatments.
Negative screening: Screen which genes are more sensitive to different treatments.
Principle
After predicting the target gene and designing the gRNA sequences, synthesize the gRNA sequences by microarray. The lentiviral vector is used to transfect all the gRNAs into the target cells (low MOI value). High-throughput sequencing technology is used to detect the changes of gRNA abundance in cells before and after treatment, and to infer the corresponding relationship between gene function and cell phenotype.
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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory