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Cat. No.: T7TMS-5k (for 5KU)

Cat. No.: T7TMS-25k (for 25KU)

For standard version, please visit T7 RNA Polymerase.

Features

Compared with wild-type phage T7 RNA polymerase, T7 Thermostable RNA polymerase can transcribe in vitro at a higher temperature. It can efficiently transcribe in vitro at 50°C, while the wild type has no activity at this temperature. Based on this heat-resistant in vitro transcription reaction characteristics, it has the following advantages: 

• Improving the transcription efficiency of RNA with high GC content
• Improving the synthesis ability of long segment RNA
• Improving the capping efficiency of co-transcription when using cap analogs 
• Reducing the formation of dsRNA by-products and the immunogenicity of synthetic RNA
• Improving the detection performance of NASBA and CRISPR nucleic acid amplification

Applications

• Radiolabeled RNA probe preparation
• RNA generation for studies of the RNA structure, processing, and catalysis
• Expression control via anti-sense RNA
• mRNA, sgRNA synthesis

Storage

Store at -20°C or -80°C.

Instruction: Protocol

Related: High Yield T7 Thermostable RNA Synthesis Kit, RNasin (RNase inhibitor), NTP, DNase I (RNase Free), Thermostable Inorganic Pyrophosphatase

SBS Genetech is recognized as one of the global major leading industry players in mRNA Raw Material by third-party market researchers. For more details, please visit Top 5 mRNA Vaccine & Therapeutics Raw Material Market Companies in Global Market 2022.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory