All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: T7TRSK-10 (for 10T)
Cat. No.: T7TRSK-50 (for 50T)
Cat. No.: T7TRSK-200 (for 200T)
Description
High Yield T7 Thermostable RNA Synthesis Kit can efficiently synthesize various types of RNA in vitro, such as mRNA, lncRNA, shRNA, etc. T7 Thermostable RNA polymerase can recognize T7 promoter (TAATACGACTCA CTATA G G) and start transcription from the second to last G base. The kit can efficiently transcribe trace samples, and up to 180-200 μg transcripts can be obtained in a single reaction. The transcriptional length is more than 5000nt.
With this ready-to-use kit, the reaction can be established rapidly. This kit is also equipped with DNase I, which can quickly remove the DNA template after RNA synthesis, obtaining high-purity transcriptional RNA.
Features
Compared with wild-type phage T7 RNA polymerase, T7 Thermostable RNA Polymerase in this kit can transcribe in vitro at a higher temperature. It can efficiently transcribe in vitro at 37-52°C. The optimal temperature is 37°C. It still retains more than 50% of its activity at 50°C, while the wild type has no activity at this temperature. Based on this heat-resistant in vitro transcription reaction characteristics, it has the following advantages:
Applications
Storage
The minimum shelf life is 2 years at -20°C.
Instruction: Protocol
Related: T7 Thermostable RNA Polymerase, RNasin (RNase inhibitor), NTP, DNase I (RNase Free), Thermostable Inorganic Pyrophosphatase
Only for research and not intended for treatment of humans or animals
Journals Using SBS Genetech Products Universities Using SBS Genetech Products
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory