Cat. No.: DN1-1k (for 1KU)
Cat. No.: DN1-10k (for 10KU)
Using calf thymus DNA as substrate, under the condition of 25°C and pH 5.0, the enzyme amount needed to increase the 260 nm absorbance of the reaction solution by 0.001 in 1 minute is defined as one active unit (Kunitz unit).
Minimum shelf life is 2 years under -20°C.
Generally, DNase I can be inactivated by heating. If it is necessary to remove the residual denatured protein, the RNA sample can be extracted and precipitated with phenol chloroform after digestion at 37°C (without heating).
- 10 × DN Stop Buffer contains chelating agent to remove divalent cations. Before heat inactivation, 5 μL 10 × DN Stop Buffer must be added and mixed evenly, and then heat inactivation is carried out. Otherwise, it will lead to RNA degradation.
- When the final RNA sample is processed for one-step reverse transcription reaction, the addition amount should be less than 20% (for example, the addition amount in 20 μl reverse transcription system should be less than 4 μL)
Only for research and not intended for treatment of humans or animals
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