DNase I, RNase-free (GMP)
$500.00 - $2,500.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: DNIGMP-10k (for 10KU)
Cat. No.: DNIGMP-100k (for 100KU)
Description
DNase I, RNase-free (GMP), is an endodeoxyribonuclease that catalyzes the degradation of both single- and double-stranded DNA randomly, yielding 5'-phosphate terminal oligonucleotides. Its activity is contingent upon Ca2+ but can be further potentiated by bivalent metal ions such as Mg2+ and Mn2+. In the presence of Mg2+, the enzyme exhibits the capability to randomly cleave any site on either strand of double-stranded DNA. Conversely, in the presence of Mn2+, DNase I demonstrates a higher affinity for nearly identical sites on both strands of DNA, resulting in the generation of DNA fragments with either blunt ends or sticky ends, characterized by 1 to 2 nucleotide overhangs.
DNase I, RNase-free (GMP) is the GMP-compliant version.
Definition of Activity
One active unit is defined as the amount of enzyme which will completely degrade 1 µg of pUC19 DNA in 10 minutes at 37℃ in DNase I Reaction Buffer.
Application
- Elimination of contaminating genomic DNA from RNA samples.
- Degradation of DNA templates in transcription reactions.
- DNase I footprinting analysis.
- Nick translation for DNA labeling.
- Construction of DNA libraries for diverse applications.
Quality Control
- Protein Purity: The enzyme is ≥95% pure as determined by SDS-PAGE analysis using Coomassie Blue staining.
- RNase Activity: A 10 µl reaction containing 500 ng of total RNA and 1 U of DNase I, RNase-free incubated for 1 hours at 37℃ results in >90% of the substrate RNA remains intact as determined by agarose gel electrophoresis.
Storage
-20°C.
Related:
Only for research and not intended for treatment of humans or animals
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