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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
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  • …  
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      • All Products
      • Custom Services
      • Catalog Products
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      • Nucleic Acid Related
      • Natural Compounds
      • Synthetic Biology
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    • POCT Solution 
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      • Lateral Flow System
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DNase I, RNase-free (GMP)

DNase I, RNase-free (GMP)

$500.00 - $2,500.00
DNase I, RNase-free (GMP), is an endodeoxyribonuclease that catalyzes the degradation of both single- and double-stranded DNA randomly, yielding 5'-phosphate terminal oligonucleotides. Its activity is contingent upon Ca2+ but can be further potentiated by bivalent metal ions such as Mg2+ and Mn2+. In the presence of Mg2+, the enzyme exhibits the capability to randomly cleave any site on either strand of double-stranded DNA. Conversely, in the presence of Mn2+, DNase I demonstrates a higher affinity for nearly identical sites on both strands of DNA, resulting in the generation of DNA fragments with either blunt ends or sticky ends, characterized by 1 to 2 nucleotide overhangs.
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All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: DNIGMP-10k (for 10KU)

Cat. No.: DNIGMP-100k (for 100KU)

 

 

Description

DNase I, RNase-free (GMP), is an endodeoxyribonuclease that catalyzes the degradation of both single- and double-stranded DNA randomly, yielding 5'-phosphate terminal oligonucleotides. Its activity is contingent upon Ca2+ but can be further potentiated by bivalent metal ions such as Mg2+ and Mn2+. In the presence of Mg2+, the enzyme exhibits the capability to randomly cleave any site on either strand of double-stranded DNA. Conversely, in the presence of Mn2+, DNase I demonstrates a higher affinity for nearly identical sites on both strands of DNA, resulting in the generation of DNA fragments with either blunt ends or sticky ends, characterized by 1 to 2 nucleotide overhangs.

DNase I, RNase-free (GMP) is the GMP-compliant version.

 

Definition of Activity

One active unit is defined as the amount of enzyme which will completely degrade 1 µg of pUC19 DNA in 10 minutes at 37℃ in DNase I Reaction  Buffer.

 

Application

  • Elimination of contaminating genomic DNA from RNA samples.
  • Degradation of DNA templates in transcription reactions.
  • DNase I footprinting analysis.
  • Nick translation for DNA labeling.
  • Construction of DNA libraries for diverse applications.

 

Quality Control

  • Protein Purity: The enzyme is ≥95% pure as determined by SDS-PAGE analysis using Coomassie Blue staining.
  • RNase Activity: A 10 µl reaction containing 500 ng of total RNA and 1 U of DNase I, RNase-free incubated for 1 hours at 37℃ results in >90% of the substrate RNA remains intact as determined by agarose gel electrophoresis.

 

Storage

-20°C.

 

Comparison

DNase I, RNase-free (Lyophilized)

 

Related:

  • DNase I, RNase-free (from Bovine pancreas)
  • DNase I, RNase-free (GMP) (from yeast)
  • DNase I, RNase-free (Lyophilized) (from yeast)
  • DNase I-ST, RNase-free (from yeast)

 

 

SBS Genetech is recognized as one of the global major leading industry players in mRNA Raw Material by third-party market researchers. For more details, please visit Top 5 mRNA Vaccine & Therapeutics Raw Material Market Companies in Global Market 2022.

 

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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