Cat. No.: HLDN-100 (for 100U)
Cat. No.: HLDN-1k (for 1,000U)
Features
Components
Unit definition
One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml of high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl2.
Heat Inactivation
HL-dsDNase can be heat inactivated by treatment at 55°C for 5 min.
Storage
Minimum shelf life is 3 years at -20°C.
Notes
1×dsDNase Buffer:20mM Tris-HCl pH8, 2mM MgCl2. The enzyme needs 1 - 3 mM MgCl2.
The enzyme is sensitive to DTT. Therefore, DTT should be avoided in any reaction system.
Generally, the dosage of the enzyme in RT reaction is 0.1 - 0.5u/20 μL system.
SDS, guanidine hydrochloride, β - mercaptoethanol or high salt ions (> 300 mm) will inhibit the activity of the enzyme.
The optimal reaction temperature of the enzyme is 25 - 37 ℃, and the activity will lose 70% after 30 min at 42 ℃.