Cat. No.: HLDN-100 (for 100U)
Cat. No.: HLDN-1k (for 1,000U)
- Recommended protocol for gDNA removal from RNA preps
- Compatible with the reaction buffers of M-MLV and AMV
- Ideal for reverse transcription reaction to remove gDNA and improve the efficiency
One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml of high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl2.
HL-dsDNase can be heat inactivated by treatment at 55°C for 5 min.
Minimum shelf life is 3 years at -20°C.
1×dsDNase Buffer：20mM Tris-HCl pH8, 2mM MgCl2. The enzyme needs 1 - 3 mM MgCl2.
The enzyme is sensitive to DTT. Therefore, DTT should be avoided in any reaction system.
Generally, the dosage of the enzyme in RT reaction is 0.1 - 0.5u/20 μL system.
SDS, guanidine hydrochloride, β - mercaptoethanol or high salt ions (> 300 mm) will inhibit the activity of the enzyme.
The optimal reaction temperature of the enzyme is 25 - 37 ℃, and the activity will lose 70% after 30 min at 42 ℃.
Only for research and not intended for treatment of humans or animals