With T7 phage promoter and template DNA sequence, T7 RNA polymerase can synthesize RNA in vitro. Both linear blunt end and 5' sticky end of double-stranded DNA can be used as the template, so the linear plasmids and PCR products are ideal for the reaction. Whether the synthesized RNA strand is sense or antisense depends on the relative position of the template DNA sequence and T7 promoter. When the template DNA sequence is downstream of the T7 promoter, T7 RNA polymerase will transcribe the sense RNA strand. Otherwise, it will transcribe antisense RNA strands.
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