Cat. No.: TIPP-2k (for 2,000U)
Thermostable Inorganic Pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate: P2O7-4 + H2O -> 2HPO4-2. This enzyme has 100% activity when heated at 100°C for 4 h, so it can be used in PCR reaction to remove the inhibition of inorganic pyrophosphate on PCR and enhance DNA replication.
One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).
- The enzyme is active in a variety of reaction buffers. Therefore, it can be directly used in tHDA amplification, PCR and sequencing.
- The dosage of the enzyme needs to be optimized in different experiments, usually in the concentration of 5 - 50U/ml.
- The enzyme could not be inactivated by heat.
-20°C for storage with a shelf life of 3 years.
Only for research and not intended for treatment of humans or animals
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