Thermostable Inorganic Pyrophosphatase was purified from recombinant E. coli strain, which carried the gene of inorganic pyrophosphatase cloned from Thermococcus litoralis. The enzyme catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate.
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Cat. No.: TIPP-2k (for 2,000U)
Thermostable Inorganic Pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate: P2O7-4 + H2O -> 2HPO4-2. This enzyme has 100% activity when heated at 100°C for 4 h, so it can be used in PCR reaction to remove the inhibition of inorganic pyrophosphate on PCR and enhance DNA replication.
One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10-minute reaction at 75°C in 50 mM Tricine [pH 8.5], 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).
The enzyme is active in a variety of reaction buffers. Therefore, it can be directly used in thermophilic helicase-dependent amplification (tHDA), PCR, and sequencing.
The dosage of the enzyme needs to be optimized in different experiments, usually in the concentration of 5 - 50U/ml.
The enzyme could not be inactivated by heat.
The minimum shelf life is 3 years at -20°C.
Only for research and not intended for treatment of humans or animals
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