Cat. No.: FMM-10K
MMLV Reverse Transcriptase is able to synthesize the complementary DNA strand from a single-strand RNA template. M-MLV Reverse Transcriptase (RNase H-) is a mutant type of M-MLV Reverse Transcriptase, obtained by eliminating the active center of RNase H through multiple point mutations. The alteration decreases activity of RNase H and reduces RNA degradation in reverse transcription, which increases the yield of first-strand cDNA to get full length cDNA more easily. In addition, thermal stability of the reverse transcriptase is improved and the optimal reaction temperature is therefore 50℃.
Source and Application:
The enzyme is obtained from E.coli strain containing mutant Molony murine leukemia virus (M-MLV) reverse transcriptase gene.It could be applied in first-strand cDNA synthesis and RT-PCR
Instruction: M-MLV User's Instruction
Only for research and not intended for treatment of humans or animals