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Cat. No.: WAMM-10k (for 10KU) 

For M-MLV Reverse Transcriptase active at 72°C, please see Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-).

For M-MLV Reverse Transcriptase active at 65°C, please see Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-).

For M-MLV Reverse Transcriptase active at 55°C, please see Thermo-stable M-MLV Reverse Transcriptase (RNase H-).

Description

Warm Activated Reverse Transcriptase is a RNase H-deficient variant of Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is cloned and expressed through gene modification and recombinant techniques. It employs nucleic acid adapter technology to covalently bind to M-MLV, effectively inhibiting its activity at temperatures below 31°C, thus enhancing product specificity. Through genetic engineering modifications, the enzyme's optimal reaction temperature has been raised to 50°C, providing improved extension capability, making it suitable for longer cDNA synthesis.

Components

• Warm Activated Reverse Transcriptase (200 U/μl): 50 μl
• 5× M-MLV First Strand Buffer: 500 μl
• 0.1 M DTT: 100 μl

Features

• Warm Activation: This reverse transcriptase remains inhibited at lower temperatures (below 31°C), but its activity is enhanced at 50°C, making it suitable for reactions at higher temperatures.
• RNase H Deficient: This reverse transcriptase is a variant with RNase H activity deficiency, which helps prevent RNA degradation, improving the efficiency and stability of cDNA synthesis.
• Utilizes Nucleic Acid Adapter Technology: It covalently binds to M-MLV using nucleic acid adapter technology, increasing its specificity and reducing the risk of nonspecific synthesis.
• Enhanced Product Specificity: By inhibiting M-MLV activity at lower temperatures, this enzyme enhances product specificity, aiding in obtaining more accurate cDNA products.
• Greater Extension Capability: Through genetic engineering modifications, the optimal reaction temperature of this reverse transcriptase has been raised to 50°C, enhancing its extension capability, making it suitable for longer cDNA synthesis reactions.

Related: 

• Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
• Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
• Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
• TGI-RTase Reverse Transcriptase
• Warm Activated Reverse Transcriptase (10KU)

Only for research and not intended for treatment of humans or animals

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