thumbnail image
tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
    • Product 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Natural Compounds
      • Synthetic Biology
      • Enzymes
    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Freeze-Drying System
      • Lateral Flow System
    • About 
      • About SBS
      • Achievements
      • Ecosystem
      • Legal Statement
    • Contact
    • Login
tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
    • Product 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Natural Compounds
      • Synthetic Biology
      • Enzymes
    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Freeze-Drying System
      • Lateral Flow System
    • About 
      • About SBS
      • Achievements
      • Ecosystem
      • Legal Statement
    • Contact
    • Login
Beijing SBS Genetech Co.,Ltd.
Go Back
Warm Activated Reverse Transcriptase (10KU)

Warm Activated Reverse Transcriptase (10KU)

$400.00
Warm Activated Reverse Transcriptase is a RNase H-deficient variant of Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is cloned and expressed through gene modification and recombinant techniques. It employs nucleic acid adapter technology to covalently bind to M-MLV, effectively inhibiting its activity at temperatures below 31°C, thus enhancing product specificity. Through genetic engineering modifications, the enzyme's optimal reaction temperature has been raised to 50°C, providing improved extension capability, making it suitable for longer cDNA synthesis.
Quantity
Coming soon
Add to cart
More Details

All products have special prices for bulk purchase, please contact us for more details if required.

 

Cat. No.: WAMM-10k (for 10KU) 

 

For M-MLV Reverse Transcriptase active at 72°C, please see Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-).

For M-MLV Reverse Transcriptase active at 65°C, please see Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-).

For M-MLV Reverse Transcriptase active at 55°C, please see Thermo-stable M-MLV Reverse Transcriptase (RNase H-).

 

Description

Warm Activated Reverse Transcriptase is a RNase H-deficient variant of Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is cloned and expressed through gene modification and recombinant techniques. It employs nucleic acid adapter technology to covalently bind to M-MLV, effectively inhibiting its activity at temperatures below 31°C, thus enhancing product specificity. Through genetic engineering modifications, the enzyme's optimal reaction temperature has been raised to 50°C, providing improved extension capability, making it suitable for longer cDNA synthesis.

 

Components

  • Warm Activated Reverse Transcriptase (200 U/μl): 50 μl
  • 5× M-MLV First Strand Buffer: 500 μl
  • 0.1 M DTT: 100 μl

 

Features

  • Warm Activation: This reverse transcriptase remains inhibited at lower temperatures (below 31°C), but its activity is enhanced at 50°C, making it suitable for reactions at higher temperatures.
  • RNase H Deficient: This reverse transcriptase is a variant with RNase H activity deficiency, which helps prevent RNA degradation, improving the efficiency and stability of cDNA synthesis.
  • Utilizes Nucleic Acid Adapter Technology: It covalently binds to M-MLV using nucleic acid adapter technology, increasing its specificity and reducing the risk of nonspecific synthesis.
  • Enhanced Product Specificity: By inhibiting M-MLV activity at lower temperatures, this enzyme enhances product specificity, aiding in obtaining more accurate cDNA products.
  • Greater Extension Capability: Through genetic engineering modifications, the optimal reaction temperature of this reverse transcriptase has been raised to 50°C, enhancing its extension capability, making it suitable for longer cDNA synthesis reactions.

 

Related: 

  • Mutant M-MLV Reverse Transcriptase
  • Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
  • Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
  • Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
  • TGI-RTase Reverse Transcriptase
  • Warm Activated Reverse Transcriptase

 

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

  • background image
    background image
    background image
    background image
    background image
    background image
    background image
  • Featured Publications

    We are honored to create value for our customers and facilitate the development of science

SBS Genetech © Copyright 2000-2025

from China, for the World

for Superior Biology Services since 2000

    Home
    Journals
    Contact
    Posts
Cookie Use
We use cookies to ensure a smooth browsing experience. By continuing we assume you accept the use of cookies.
Learn More