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Cat. No.: FUMM-20k (for 20KU) 

Cat. No.: FUMM-100k (for 100KU) 

For M-MLV Reverse Transcriptase active at 55°C, please see Thermo-stable M-MLV Reverse Transcriptase (RNase H-).

For M-MLV Reverse Transcriptase active at 72°C, please see Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-).

For M-MLV Reverse Transcriptase with DNA polymerase activity, please see DPolUltra Thermo-stable M-MLV Reverse Transcriptase.

The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system.

Description

Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-) is a mutant type of M-MLV Reverse Transcriptase, obtained by eliminating the active center of RNase H through multiple point mutations. The alteration decreases the activity of RNase H and reduces RNA degradation in reverse transcription, which increases the yield of first-strand cDNA and gets full-length cDNA more easily. 

In addition, the thermal stability of the reverse transcriptase is improved and the enzyme is active up to 65°C. Compared with RT-PCR at low temperature, RT-PCR at a high temperature can significantly open the secondary structure of RNA, promote the amplification performance of complex RNA templates, increase the length and yield of cDNA, and improve the sensitivity of subsequent detection.

This product has a high concentration (200U/μl) and is glycerol-free, which can be used to establish a freeze-drying system.

Unit Definition

One active unit is defined as the amount of enzyme needed to catalyze the incorporation 1 nmol dTTP into acid-insoluble sediment in 10 min under 37°C with poly(rA) as template and oligo(dT) as primer.

Features

RT-PCR at high temperature will help you:

• Open the secondary structure of RNA
• Promote the amplification performance of complex RNA template
• Increase the length and yield of cDNA
• Improve the sensitivity of subsequent detection

Storage

The minimum shelf life is 1 month at 4°C, 1 year at -20°C, and 3 years at -80°C.

Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.

Instruction: Protocol

Related: 

• Mutant M-MLV Reverse Transcriptase
• Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
• Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
• Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
• TGI-RTase Reverse Transcriptase
• Warm Activated Reverse Transcriptase

Only for research and not intended for treatment of humans or animals

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