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Description

The Pichia pastoris Host Cell Residual DNA Detection Kit is specifically designed for the quantitative analysis of residual P. pastoris host cell DNA in intermediates, semi‑finished, and finished biological products.

This kit applies probe‑based quantitative fluorescence PCR (qPCR) to accurately measure residual P. pastoris DNA in samples. It features high specificity, rapid detection, stable performance, and a detection limit down to the femtogram (fg) level.

A P. pastoris DNA quantitative reference standard is included, enabling precise quantification of residual DNA in test samples.

Kit Components

• P. pastoris qPCR Mix  
• P. pastoris Primer & Probe Mix 
• P. pastoris DNA Quantitative Reference (30 ng/μL)  
• DNA Dilution Buffer

Note: This kit does not contain ROX reference dye. If your qPCR instrument requires ROX, it must be purchased separately.

Shelf Life

Stable for 2 years when stored under the recommended conditions. For details, please refer to the product label.

Compatible Instruments (including but not limited to)

• Thermo Scientific: ABI 7500, QuantStudio™ 5, ABI StepOnePlus
• Bio-Rad: CFX96

Required Equipment and Materials

• Instruments: Pipettes, vortex mixer, microcentrifuge, qPCR instrument
• Consumables: EP tubes, PCR tubes, 8-strip tubes, sterile pipette tips

Precautions

• Please read this manual carefully before use and follow the instructions strictly.
• Mix each component thoroughly and briefly centrifuge before use.
• Perform sample preparation and reagent setup in separate areas of a clean bench.
• Return reagents to the recommended storage conditions promptly after use; avoid prolonged exposure to room temperature or high temperatures.
• Ensure all consumables (EP tubes, PCR tubes, pipette tips, etc.) are sterile and nuclease-free.

Related:

• Universal Magnetic Bead–Based Host Residual DNA Sample Pretreatment Kit
• E. coli Host Cell Residual DNA Detection Kit
• CHO Host Cell Residual DNA Detection Kit
• Vero Host Cell Residual DNA Detection Kit
• Pichia pastoris Host Cell Residual DNA Detection Kit
• Hansenula Host Cell Residual DNA Detection Kit
• Vero Host Cell Residual DNA Fragment Analysis Kit
• HEK293 Host Cell Residual DNA Detection Kit
• Clostridium botulinum Host Cell Residual DNA Detection Kit
• HEK293 Host Cell Residual DNA Fragment Analysis Kit
• Saccharomyces cerevisiae Host Cell Residual DNA Detection Kit

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Only for research and not intended for treatment of humans or animals