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Description

The HEK293 Host Cell Residual DNA Fragment Analysis Kit is designed for the quantitative analysis of residual DNA fragment size distribution from HEK293 host cells in intermediates, semi-finished, and finished biological products.

This kit applies probe-based quantitative fluorescence PCR (qPCR), with primer–probe sets targeting four fragment lengths (80 bp, 147 bp, 267 bp, and 381 bp). It enables accurate quantification of the size distribution of residual HEK293 DNA fragments in samples. The kit offers high specificity, rapid detection, stable performance, and a detection limit down to the femtogram (fg) level.

A specially formulated lysis buffer is included, developed for recombinant vector products. For samples such as AAV, LV, and AdV, DNA extraction and purification are not required—simple dilution and lysis are sufficient for direct use as PCR templates.

The kit also incorporates a dUTP/UDG anti-contamination system, effectively eliminating contamination from PCR amplicon aerosols.

Kit Components

• HEK293 qPCR Mix
• HEK293 Primer & Probe Mix‑80
• HEK293 Primer & Probe Mix‑147 
• HEK293 Primer & Probe Mix‑267  
• HEK293 Primer & Probe Mix‑381 
• HEK293 DNA Quantitative Reference (30 ng/μL)   
• DNA Dilution Buffer
• Lysis Buffer

Note: This kit does not contain ROX reference dye. If your qPCR instrument requires ROX, it must be purchased separately.

Shelf Life

Stable for 1 year when stored under the recommended conditions. For details, please refer to the product label.

Compatible Instruments (including but not limited to)

• Thermo Scientific: ABI 7500, QuantStudio™ 5, ABI StepOnePlus
• Bio-Rad: CFX96

Required Equipment and Materials

• Instruments: Pipettes, vortex mixer, microcentrifuge, qPCR instrument
• Consumables: EP tubes, PCR tubes, 8-strip tubes, sterile pipette tips

Precautions

• Please read this manual carefully before use and follow the instructions strictly.
• Mix each component thoroughly and briefly centrifuge before use.
• Perform sample preparation and reagent setup in separate areas of a clean bench.
• Return reagents to the recommended storage conditions promptly after use; avoid prolonged exposure to room temperature or high temperatures.
• Ensure all consumables (EP tubes, PCR tubes, pipette tips, etc.) are sterile and nuclease-free.

Related:

• Universal Magnetic Bead–Based Host Residual DNA Sample Pretreatment Kit
• E. coli Host Cell Residual DNA Detection Kit
• CHO Host Cell Residual DNA Detection Kit
• Vero Host Cell Residual DNA Detection Kit
• Pichia pastoris Host Cell Residual DNA Detection Kit
• Hansenula Host Cell Residual DNA Detection Kit
• Vero Host Cell Residual DNA Fragment Analysis Kit
• HEK293 Host Cell Residual DNA Detection Kit
• Clostridium botulinum Host Cell Residual DNA Detection Kit
• HEK293 Host Cell Residual DNA Fragment Analysis Kit
• Saccharomyces cerevisiae Host Cell Residual DNA Detection Kit

Due to continuous innovation in molecular detection technology, SBS Genetech has been recognized as a Top Pharma and Life Science Technology Company by APAC CIO Outlook. For more details, please visit SBS Genetech Won APAC CIOoutlook "Top Pharma and Life S....

Only for research and not intended for treatment of humans or animals