SD DNA Polymerase is a novel artificial thermostable polymerase with strong strand displacement activity, but lacking 5′-3′ exonuclease activity. Unlike natural enzymes with strong strand displacement activity such as Phi29 or Bst polymerase, which are active only below 68 °C, SD DNA polymerase is stable up to 90 °C. Therefore, SD DNA polymerase is particularly effective for PCDR, a new PCR assay that incorporates strand displacement, particularly useful in creating more sensitive qPCRs. The amplification rate is 4 kb/min.
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SD DNA Polymerase is a novel artificial thermostable polymerase with strong strand displacement activity. SD DNA polymerase is stable up to 90°C. Therefore, SD DNA polymerase is particularly effective for PCDR (Polymerase Chain Displacement Reaction), a new PCR assay that incorporates strand displacement, particularly useful in creating more sensitive qPCRs. In addition, the enzyme can be used for the amplification of LAMP, tHDA, RCA, and library.
The enzyme has 5 '- 3' polymerase activity, and 5 '- 3' strand displacement activity. The enzyme has no 5 '- 3' exonuclease activity. The extension rate is above 4kb/min, with the best activity at 68°C. With electronic reframing technology, SD DNA Polymerase has improved its tolerance to ethanol, guanidine salt, heparin, serum, and plant polysaccharide polyphenols.
Strong strand displacement activity and polymerase activity.
Stable at high temperatures.
Tolerable to ethanol, guanidine salt, heparin, serum, and plant polysaccharide polyphenols.
Ideal for long and complex template amplification.
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into acid-insoluble substances in 30 min at 68 °C.
The minimum shelf life is 2 years at -20°C.
Polymerase chain displacement reaction (PCDR) uses multiple nested primers in a rapid, capped, one-tube reaction that increases the sensitivity of normal quantitative PCR (qPCR) assays. In PCDR, when extension occurs from the outer primer, it displaces the extension strand produced from the inner primer by utilizing a polymerase that has strand displacement activity. This allows a greater than 2-fold increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Increased sensitivity in PCDR would be useful in nucleic acid detection for viral diagnostics.