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SD DNA Polymerase (1,000U)
SD DNA Polymerase is a novel artificial thermostable polymerase with strong strand displacement activity, but lacking 5′-3′ exonuclease activity. Unlike natural enzymes with strong strand displacement activity such as Phi29 or Bst polymerase, which are active only below 68 °C, SD DNA polymerase is stable up to 90 °C. Therefore, SD DNA polymerase is particularly effective for PCDR, a new PCR assay that incorporates strand displacement, particularly useful in creating more sensitive qPCRs. The amplification rate is 4 kb/min.
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Cat. No.: SDP-1k
For the hot-start version, please see SD HS DNA Polymerase.
Description
SD DNA Polymerase is a novel artificial thermostable polymerase with strong strand displacement activity. SD DNA polymerase is stable up to 90°C. Therefore, SD DNA polymerase is particularly effective for PCDR (Polymerase Chain Displacement Reaction), a new PCR assay that incorporates strand displacement, particularly useful in creating more sensitive qPCRs. In addition, the enzyme can be used for the amplification of LAMP, tHDA, RCA, and library.
The enzyme has 5 '- 3' polymerase activity, and 5 '- 3' strand displacement activity. The enzyme has no 5 '- 3' exonuclease activity. The extension rate is above 4kb/min, with the best activity at 68°C. With electronic reframing technology, SD DNA Polymerase has improved its tolerance to ethanol, guanidine salt, heparin, serum, and plant polysaccharide polyphenols.
Features
Strong strand displacement activity and polymerase activity.
Stable at high temperatures.
Tolerable to ethanol, guanidine salt, heparin, serum, and plant polysaccharide polyphenols.
Ideal for long and complex template amplification.
Components
Unit definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into acid-insoluble substances in 30 min at 68 °C.
Storage
The minimum shelf life is 2 years at -20°C.
About PCDR
Polymerase chain displacement reaction (PCDR) uses multiple nested primers in a rapid, capped, one-tube reaction that increases the sensitivity of normal quantitative PCR (qPCR) assays. In PCDR, when extension occurs from the outer primer, it displaces the extension strand produced from the inner primer by utilizing a polymerase that has strand displacement activity. This allows a greater than 2-fold increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Increased sensitivity in PCDR would be useful in nucleic acid detection for viral diagnostics.
Instruction: Protocol
SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and DataOnly for research and not intended for treatment of humans or animals
