CM HS-SD DNA Polymerase (Gen-2)
$525.00 - $1,750.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: CSDP2-500 (for 500U)
Cat. No.: CSDP2-2500 (for 2500U)
Description
CM HS-SD DNA Polymerase (Gen-2) is a novel thermostable DNA polymerase with strong strand displacement activity, combining the displacement capability and processivity of Phi29 and Bst DNA polymerases, while tolerating temperatures up to 95 °C. These features make it an ideal enzyme for Polymerase Chain Displacement Reaction (PCDR).
The enzyme exhibits 5′→3′ polymerase activity and 5′→3′ strand displacement activity, with no 5′→3′ exonuclease activity. It has an amplification rate of approximately 1 kb/min, with optimal activity at 72 °C.
CM HS-SD DNA Polymerase (Gen-2) utilizes chemical modification to suppress enzymatic activity below 50 °C, which is fully restored only after heating at 95 °C for 5 minutes. This configuration ensures high amplification specificity and is particularly well-suited for PCDR applications.
Note: Due to the required 5-minute heat activation step, this enzyme is not compatible with isothermal amplification and is therefore not recommended for LAMP reactions.
Feature
SD DNA Polymerase (Gen‑2) is an engineered variant of SD DNA Polymerase, selected and optimized for enhanced thermal stability, withstanding temperatures up to 95 °C. This increased heat tolerance enables more thorough denaturation of complex samples, thereby improving the sensitivity of Polymerase Chain Displacement Reaction (PCDR) assays for the detection of complex target genes.
Unit definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into acid‑insoluble material within 30 minutes at 70 °C.
Applications
Isothermal amplification, LAMP, heat‑denatured LAMP, tHDA, RCA, PCDR, whole‑genome sequencing, single‑cell sequencing, and NGS library preparation.
Storage
The minimum shelf life is 2 years at -20°C.
About PCDR
Polymerase chain displacement reaction (PCDR) uses multiple nested primers in a rapid, capped, one-tube reaction that increases the sensitivity of normal quantitative PCR (qPCR) assays. In PCDR, when extension occurs from the outer primer, it displaces the extension strand produced from the inner primer by utilizing a polymerase that has strand displacement activity. This allows a greater than 2-fold increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Increased sensitivity in PCDR would be useful in nucleic acid detection for viral diagnostics.
Related:
- SD DNA Polymerase
- SD DNA Polymerase (Gen-2)
- AP HS-SD DNA Polymerase (Gen-2)
- CM HS-SD DNA Polymerase (Gen-2)
Comparison
Thermal Stability | SD DNA Polymerase
| SD DNA Polymerase (Gen-2)
| AP HS-SD DNA Polymerase (Gen-2)
| CM HS-SD DNA Polymerase (Gen-2)
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| Inhibition Efficiency |
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| PCDR Amplification |
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| LAMP Amplification |
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SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data
