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Cat. No.: PR004-50 (for 50 preparations)

Cat. No.: PR004-250 (for 250 preparations)

The Pure-miRNA series is our newly launched line of RNA extraction kits, comparable to the product lines of the world's top-tier companies. With equally remarkable quality at less than half the price, it's an irresistible choice to elevate your research endeavors.

Description

In recent years, there has been an urgent need for a reagent kit capable of efficiently extracting small RNA molecules, approximately 15-30 nucleotides in size (including siRNA and miRNA), due to extensive research on RNA interference and regulatory small RNAs. However, traditional RNA extraction methods such as silica membranes fail to effectively adsorb and recover these molecules, while phenol/chloroform extraction and ethanol precipitation are ineffective in precipitating and recovering small RNA molecules. This reagent kit utilizes a unique lysis solution/Beta-mercaptoethanol to rapidly disrupt cells and deactivate cellular RNAases, followed by organic extraction to remove proteins and DNA effectively. RNA, including small RNA molecules, is adsorbed onto the special silica membrane within the centrifuge column. Subsequently, through a series of rapid wash and centrifugation steps, wash solutions further remove cellular metabolites, proteins, and other impurities. Finally, low-salt elution buffer is used to elute pure RNA from the silica membrane.

Features

• All centrifuge adsorption column membranes are made of high-quality custom adsorption membranes, with minimal differences in adsorption between columns and good repeatability. This overcomes the instability of membrane quality in ordinary reagent kits.

• There is also no need for steps such as ethanol precipitation, which easily lead to loss of small RNA molecules.

• Fast, simple, with individual sample processing typically completed within 30 minutes.

• Multiple column washes ensure high purity, with a typical OD260/OD280 ratio ranging from 1.9 to 2.0 and minimal DNA residue, suitable for RNAi, RT-PCR, Northern blot, and various experiments.