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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
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      • All Products
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      • Synthetic Biology
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    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
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phi29 DNA Polymerase
  • phi29 DNA Polymerase
  • phi29 DNA Polymerase

phi29 DNA Polymerase

$156.00 - $3,120.00
$3,900.00
phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 (Φ29). This polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification. phi29 DNA Polymerase also possesses an inherent 3´→ 5' proofreading exonuclease activity, thus the fidelity of the synthesized DNA fragments is very high. 3'-modified primers are highly recommended to reduce the cleavage effect of the exonuclease activity on the primer.
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All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: P29P-1k (for 1,000U)

Cat. No.: P29P-20k (for 20 KU)

Cat. No.: P29P-100k (for 100 KU)

 

For an updated version that can continuously synthesize DNA at 42°C, please see phi29 HT DNA Polymerase.

 

The lyophilized phi29 DNA Polymerase is also available by inquiry, which can be transported at room temperature.

The high concentration (1000U/μl) version is available for this product by inquiry.

 

Features

  • Strong strand displacement capability
  • Continuous synthesis capability
  • High fidelity of amplification

 

Applications

  • DNA isothermal rolling loop amplification
  • DNA synthesis requiring strong strand displacement
  • Rapid replication of plasmids
  • Rapid replication requiring high fidelity

 

Storage

Stored at -20°C for 3 years.

 

Notes

  • The optimum reaction temperature of the enzyme is 30°C.
  • The enzyme can be inactivated at 65°C in 10 min.
  • Adjust the concentration of dNTP from 100 to 500 μM according to the type of experiments.
  • The addition of Yeast Pyrophosphatase to the reaction mixture with phi29 DNA Polymerase may enhance DNA synthesis.
  • Thio-modification at the 3' end of the primers can avoid primer degradation.
 

Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.

 

Related: 

  • phi29 DNA Polymerase
  • phi29 DNA Polymerase (glycerol-free)
  • phi29 DNA Polymerase (lyophilized)
  • phi29 HT DNA Polymerase
  • PrimeIAmp™ Rolling Circle Amplification Kit
  • PrimeIAmp™ Primase Rolling Circle Amplification (RCA) Kit
  • Tth PrimPol Primase
 
 
SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data
 
 
 
 
 
Only for research and not intended for treatment of humans or animals
 
 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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