Rolling circle amplification (RCA) is an isothermal enzymatic method developed in recent years. A short DNA primer is amplified to form a long single-stranded DNA using a circular DNA template and phi29 HT DNA Polymerase. The RCA product is a concatemer containing tens to hundreds of tandem repeats that are complementary to the circular template. The power, simplicity, and versatility of the DNA amplification technique have made it an attractive tool for biomedical research and nanobiotechnology.
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Cat. No.: IARCA-50 (for 50T)
With high-quality phi29 HT DNA Polymerase, PrimeIAmp™ Rolling Circle Amplification Kit features strong strand displacement activity and continuous synthesis capability (up to more than 70 kb). In addition, the polymerase possesses an inherent 3´→ 5' proofreading exonuclease activity, thus the fidelity of the synthesized DNA fragments is very high. The kit is equipped with random hexamers, which can amplify the target molecule exponentially (usually up to more than 10,000 times).
4xRCA Mix: 250 μl
10XRandom Hexamer: 100 μl
RNase Free H2O: 1 ml
High sensitivity: The amplification efficiency of single-primer RCA is very high, reaching 104-105 times.
Simple instrument: Only the water bath pot which can maintain a constant temperature is required.
Easy operation: Only constant temperature amplification is needed.
Wide application: The kit can be widely used in various research fields.
The minimum shelf life is 3 years at -20°C. Avoid repeated freezing and thawing.