Cat. No.: IARCA-50 (for 50T)

Description

With high-quality phi29 HT DNA Polymerase, PrimeIAmp™ Rolling Circle Amplification Kit features strong strand displacement activity and continuous synthesis capability (up to more than 70 kb). In addition, the polymerase possesses an inherent 3´→ 5' proofreading exonuclease activity, thus the fidelity of the synthesized DNA fragments is very high. The kit is equipped with random hexamers, which can amplify the target molecule exponentially (usually up to more than 10,000 times).

Content

• 4xRCA Mix: 250 μl 
• 10XRandom Hexamer: 100 μl 
• RNase Free H₂O: 1 ml

Features

• High sensitivity: The amplification efficiency of single-primer RCA is very high, reaching 10⁴-10⁵ times.
• Simple instrument: Only the water bath pot which can maintain a constant temperature is required.
• Easy operation: Only constant temperature amplification is needed.
• Wide application: The kit can be widely used in various research fields.

Storage

The minimum shelf life is 3 years at -20°C. Avoid repeated freezing and thawing.

Instruction: Protocol

Learn more: Choosing Your Best Phi29 DNA Polymerase and Padlock Primers to Start Your Rolling Circle Amplification

Related: phi29 HT DNA Polymerase

Only for research and not intended for treatment of humans or animals

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory