All products have special prices for bulk purchase, please contact us for more details if required.

Cat. No.: MSMM-10k (for 10KU) 

For M-MLV Reverse Transcriptase active at 72°C, please see Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-).

For M-MLV Reverse Transcriptase active at 65°C, please see Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-).

For M-MLV Reverse Transcriptase active at 55°C, please see Thermo-stable M-MLV Reverse Transcriptase (RNase H-).

Description

M-MLV SIII Reverse Transcriptase is the third generation of M-MLV reverse transcriptase obtained through genetic modification and recombinant technology. This enzyme, in comparison to the wild-type M-MLV reverse transcriptase, has eliminated RNase H activity and significantly improved thermal stability (optimal reaction temperature at 50°C). As a result, there is a substantial increase in specificity and length when synthesizing the first strand cDNA (up to 18 kb), and enhanced tolerance to the complex secondary structures of RNA.

Components

M-MLV SIII Reverse Transcriptase(200 U/μl): 50 μl

5× M-MLV First Strand Buffer: 500 μl

0.1 M DTT: 100 μl

Enzyme Activity Unit Definition

Under 37°C conditions, using Poly(A)-Oligo(dT) as a template/primer, the enzyme quantity required to incorporate 1 nmol of [³H] dTTP within 10 minutes is defined as one unit of enzyme activity (U).

Instruction: Protocol

Related: 

• Mutant M-MLV Reverse Transcriptase
• Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
• Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
• Prime Thermo-stable M-MLV Reverse Transcriptase (RNase H-)
• TGI-RTase Reverse Transcriptase
• Warm Activated Reverse Transcriptase

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory