CRISPR Cas12a-RPA DNA Lyophilized Detection Kit (One-Step)
$1,185.00 - $4,150.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: C12ALDO-96 (for 96T)
Cat. No.: C12ALDO-480 (for 480T)
Description
Nonspecific signals are a common challenge in isothermal amplification. The CRISPR/Cas system significantly enhances specificity. This kit integrates isothermal amplification with CRISPR/Cas12a to enable highly sensitive and specific DNA detection. All reactions are carried out in a single tube, in one step, and at a constant temperature. The kit includes universal reagents for both isothermal amplification and CRISPR/Cas12a-based detection, streamlining the development of point-of-care diagnostic products.
Kit Component
- Reaction Buffer (2X)
- Reaction Tube
- Starter (10X)
- Cas12a Protein
- Reporter
- Positive Control (primer, crRNA and DNA template included)
Additional Required Materials
- Template DNA
- Nuclease-free water
- qPCR thermal cycler or heat block
- Target sequence-specific primers for isothermal amplification
- crRNA/gRNA: Forms a functional complex with LbaCas12a and is specifically activated by the target sequence. (LbaCas12a crRNA scaffold sequence: 5’-UAAUUUCUACUAAGUGUAGAU-3’)
Application
The CRISPR Cas12a DNA Detection Kit (One-Step) is used for rapid DNA detection. Simply add primers and gRNA to achieve single-tube detection of DNA pathogens.
Instruction: Protocol
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