Broadonase Super Nuclease (≥99%, with His‑tag)
$45.00 - $4,700.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: BSNH-5k (for 5KU)
Cat. No.: BSNH-25k (for 25KU)
Cat. No.: BSNH-100k (for 100KU)
Cat. No.: BSNH-500k (for 500KU)
Cat. No.: BSNH-2m (for 2000KU)
Description
Broadonase Super Nuclease (≥99%, with His‑tag) is a non‑specific endonuclease derived from Serratia marcescens. It degrades a wide variety of nucleic acid substrates—including single‑stranded, double‑stranded, linear, circular, native, or denatured DNA and RNA—under broad reaction conditions, generating 5'-monophosphate oligonucleotides of 3–5 bases in length. This nuclease is widely used for nucleic acid removal in recombinant proteins, viral vaccines, and other biological products, as well as for reducing viscosity in cell, tissue, or microbial lysates. The enzyme carries an N‑terminal His‑tag, enabling removal by nickel affinity chromatography when required.
This product is ultrapure, with ≥99% purity, no detectable protease activity, and extremely low endotoxin levels (<0.25 EU/KU).
Broadonase Super Nuclease—also known as universal nuclease or broad‑spectrum nuclease—shares an essentially identical amino acid sequence with Benzonase endonuclease, TurboNuclease, and other similar nucleases (differences are limited to N‑ or C‑terminal tags or residual amino acids). These enzymes exhibit equivalent catalytic activity and are generally interchangeable. When selecting a nuclease for downstream applications, purity and endotoxin levels should be carefully considered, as they may influence analytical results.
Broadonase Super Nuclease demonstrates excellent stability. When stored at 37°C for 3–5 weeks, the enzyme retains more than 90% of its activity. After storage at 25°C for 10 weeks, no significant loss of activity is observed.
This product is expressed and purified using the proprietary technology platform. The resulting recombinant protein is identical in amino acid sequence to the native nuclease from Serratia marcescens, with an N‑terminal His‑tag and no additional residues. Its biochemical properties match those of the natural enzyme. A tag‑free version, Broadonase Super Nuclease (≥99%), is also available.
Protein Information
Category
| Description
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Activity Range and Reaction Compatibility
| Condition | Optimal Range | Effective Range |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Recommended Usage Guidelines
| Experiment Type | Protein Sample Preparation | Non‑pharmaceutical Protein Production | Pharmaceutical Protein Production | Vaccine / Virus Production | Cell Therapy |
|---|---|---|---|---|---|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Storage
- Store at −20°C; stable for three years.
- Store at 4°C; stable for at least two months.
Notes
- Storage at −80°C is not recommended, as freeze–thaw cycles may reduce enzymatic activity.
- Mg²⁺ is an essential catalytic cofactor for Broadonase Super Nuclease. A reaction buffer containing 1–2 mM Mg²⁺ is required for full enzymatic activity.
- When working with atypical solutions—such as high‑salt buffers, acidic or alkaline conditions, or solutions containing high concentrations of detergents or denaturants—refer to the optimal and effective activity ranges. In such cases, the amount of Broadonase Super Nuclease or the incubation time should be increased accordingly.
- For research use only. Not intended for clinical diagnosis, therapeutic applications, food, or pharmaceutical use. Do not store in residential environments.
- For your safety, wear a lab coat and disposable gloves during handling.
Related
- Broadonase Super Nuclease (≥99%)
- Broadonase Super Nuclease (≥99%, with His‑tag)
- Broadonase Salt‑tolerant Super Nuclease (≥99%)
Only for research and not intended for treatment of humans or animals
Journals Using SBS Genetech Products Universities Using SBS Genetech Products
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory
