Cat. No.: EUT-500
U-Taq DNA Polymerase consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5'→3' DNA polymerase activity and lacks 3'→5' exonuclease activity. Its extension rate is 2~4 kb/min in standard condition, which is the highest among all thermostable DNA enzyme. The appropriate reaction temperature is 70~75℃, the work concentration of dNTPs is 100~300 μM, the work concentration of Mg2+ is 2~3 mM, and the suitable pH is 8.1~9.1. The enzyme generates PCR products with 3'-dA overhangs, suitable for T-A cloning. The amount of enzyme is 1~1.5unit for 20μl PCR reaction, while 2~3unit for 50μl PCR reaction. 6 kb Lambda DNA and 2.1 kb Human genomic DNA can be amplified very well at our laboratory.
- U-Taq DNA Polymerase Storage Buffer: U-Taq DNA Polymerase is supplied in 50 mM Tris-HCl (pH8.0), 100 mM NaCl, 0.1 mM EDTA,0.5 mM DTT, 1% TritonX-100, and 50% Glycerol.
- 10 x U-Taq reaction buffer: 500 mM KCI, 100 mM Tris-HCI (pH8.0), 20 mM MgCI2.
Store at -20℃ for one year. Avoid repeated freeze-thaw cycles
Only for research and not intended for treatment of humans or animals