Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-) is able to synthesize the complementary DNA strand from a single-strand RNA template. With improved thermal stability, the enzyme is active even at 65°C.
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: FUMM-10k (for 10KU)
Cat. No.: FUMM-50k (for 50KU)
The glycerol-free version is also available for this product.
Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-) is a mutant type of M-MLV Reverse Transcriptase, obtained by eliminating the active center of RNase H through multiple point mutations. The alteration decreases the activity of RNase H and reduces RNA degradation in reverse transcription, which increases the yield of first-strand cDNA to get full-length cDNA more easily.
In addition, the thermal stability of the reverse transcriptase is improved and the enzyme is active up to 65°C. Compared with RT-PCR at low temperature, RT-PCR at a high temperature can significantly open the secondary structure of RNA, promote the amplification performance of complex RNA template, increase the length and yield of cDNA, and improve the sensitivity of subsequent detection.
One active unit is defined as the amount of enzyme needed to catalyze the incorporation 1 nmol dTTP into acid-insoluble sediment in 10 min under 37°C with poly(rA) as template and oligo(dT) as primer.
RT-PCR at high temperature will help you:
Open the secondary structure of RNA
Promote the amplification performance of complex RNA template
Increase the length and yield of cDNA
Improve the sensitivity of subsequent detection
The minimum shelf life is 3 years at -20°C.
Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.
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