Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-) is able to synthesize the complementary DNA strand from a single-strand RNA template. With improved thermal stability, the enzyme is active even at 65°C. This product has a high concentration (200U/μl) and is glycerol-free, which can be used to establish a freeze-drying system.
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The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system.
Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-) is a mutant type of M-MLV Reverse Transcriptase, obtained by eliminating the active center of RNase H through multiple point mutations. The alteration decreases the activity of RNase H and reduces RNA degradation in reverse transcription, which increases the yield of first-strand cDNA and gets full-length cDNA more easily.
In addition, the thermal stability of the reverse transcriptase is improved and the enzyme is active up to 65°C. Compared with RT-PCR at low temperature, RT-PCR at a high temperature can significantly open the secondary structure of RNA, promote the amplification performance of complex RNA templates, increase the length and yield of cDNA, and improve the sensitivity of subsequent detection.
This product has a high concentration (200U/μl) and is glycerol-free, which can be used to establish a freeze-drying system.
One active unit is defined as the amount of enzyme needed to catalyze the incorporation 1 nmol dTTP into acid-insoluble sediment in 10 min under 37°C with poly(rA) as template and oligo(dT) as primer.
RT-PCR at high temperature will help you:
Open the secondary structure of RNA
Promote the amplification performance of complex RNA template
Increase the length and yield of cDNA
Improve the sensitivity of subsequent detection
The minimum shelf life is 1 month at 4°C, 1 year at -20°C, and 3 years at -80°C.
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