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Cat. No.: TSCDSS-100 (for 100ml)

Description

The Tissue Single-Cell Dissociation Stop Solution is designed to terminate tissue or cell enzymatic digestion reactions, rapidly neutralizing enzyme activity and protecting cell integrity. It is widely applied in the preparation of single-cell suspensions. This solution effectively halts enzymatic reactions induced by tissue dissociation enzymes in various animal organs (such as brain, liver, spleen, kidney, lung, and tumor tissues), thereby preventing damage from over-digestion and significantly improving the success rate and data quality of downstream experiments including flow cytometry, single-cell sequencing, and cell culture.

Features

• Serum-free formulation: No animal serum proteins; defined composition avoids interference with downstream experiments.
• Ready-to-use design: No preparation required; use directly after opening.
• Broad-spectrum applicability: Contains multiple protective agents suitable for diverse tissues and organs.
• Extended preservation: Maintains tissue integrity for up to 96 hours at 2–8 °C.

Protocol

1. After enzymatic digestion is complete, immediately add an equal volume or up to twice the volume of Stop Solution to the digestion system (adjust volume according to enzyme concentration and reaction conditions).
2. Mix gently and incubate at room temperature for 1–2 minutes to fully terminate enzyme activity.
3. Centrifuge to collect cells at 300–400 × g for 5 minutes, discard the supernatant.
4. Resuspend cells in pre-cooled PBS or culture medium for downstream applications (e.g., flow cytometry, single-cell sequencing, cell culture).

Notes

• Mix thoroughly before use. If precipitation occurs, centrifuge and use the supernatant.
• Digestion conditions vary by tissue type; pre-experiments are recommended to optimize Stop Solution volume and incubation time.
• Supplied sterile; maintain aseptic conditions during use to avoid contamination.
• For research use only.

Related:

• High-Activity Cell Cryopreservation Solution
• Red Blood Cell Lysis Buffer
• High-Efficiency Debris Removal Reagent
• Tissue Single-Cell Dissociation Stop Solution
• High-Activity Tissue Preservation Solution
• Universal Tumor Tissue Single-Cell Dissociation Enzyme
• Pancreas Tissue Single-Cell Dissociation Enzyme
• Skeletal Muscle Tissue Single-Cell Dissociation Enzyme
• Lymph Node Tissue Single-Cell Dissociation Enzyme
• Testis Tissue Single-Cell Dissociation Enzyme
• Thymus Tissue Single-Cell Dissociation Enzyme
• Neonatal Heart Tissue Single-Cell Dissociation Enzyme
• Small Intestine Tissue Single-Cell Dissociation Enzyme
• Colon Tissue Single-Cell Dissociation Enzyme
• Umbilical Tissue Single-Cell Dissociation Enzyme
• Adipose Tissue Single-Cell Dissociation Enzyme
• Retinal Tissue Single-Cell Dissociation Enzyme
• Ligament Tissue Single-Cell Dissociation Enzyme
• Embryoid Tissue Single-Cell Dissociation Enzyme
• Universal Tissue Single-Cell Dissociation Enzyme

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory