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Cat. No.: RBCLBS-100 (for 100ml)

Description

The Red Blood Cell Lysis Buffer is a simple and rapid reagent for lysing red blood cells. By exploiting differences in salt ion concentration, this buffer quickly lyses enucleated red blood cells in single-cell suspensions while leaving lymphocytes and other nucleated cells intact. Sterile-filtered and ready to use, the treated single-cell suspension samples can be applied to a wide range of routine analyses, assays, and primary cell cultures.

Features

• Serum-free formulation: No animal serum proteins; defined composition avoids interference with downstream experiments.
• Ready-to-use design: No preparation required; use directly after opening.
• Broad-spectrum applicability: Contains multiple protective agents suitable for diverse tissues and organs.
• Extended preservation: Maintains tissue integrity for up to 96 hours at 2–8 °C.

Protocol

1. Collect single-cell suspension into a 1.5 mL centrifuge tube. Centrifuge at 400 × g, 4 °C for 5 minutes, discard supernatant.
2. Resuspend cells in 1 mL of room temperature–equilibrated lysis buffer. Incubate on ice for 2–5 minutes.
3. Add 10 mL of 1× PBS containing 0.2% BSA to terminate the reaction. Centrifuge at 400 × g, 4 °C for 5 minutes, discard supernatant.
4. Resuspend cells in 1 mL of 1× PBS containing 0.2% BSA.
5. Perform cell counting and proceed with downstream experiments.

Notes

• Equilibrate buffer to room temperature before use.
• If red precipitate remains after lysis, repeat treatment with buffer for 3–4 minutes.
• Not suitable for lysing nucleated red blood cells.
• For research use only, not for clinical diagnosis.

Related:

• High-Activity Cell Cryopreservation Solution
• Red Blood Cell Lysis Buffer
• High-Efficiency Debris Removal Reagent
• Tissue Single-Cell Dissociation Stop Solution
• High-Activity Tissue Preservation Solution
• Universal Tumor Tissue Single-Cell Dissociation Enzyme
• Pancreas Tissue Single-Cell Dissociation Enzyme
• Skeletal Muscle Tissue Single-Cell Dissociation Enzyme
• Lymph Node Tissue Single-Cell Dissociation Enzyme
• Testis Tissue Single-Cell Dissociation Enzyme
• Thymus Tissue Single-Cell Dissociation Enzyme
• Neonatal Heart Tissue Single-Cell Dissociation Enzyme
• Small Intestine Tissue Single-Cell Dissociation Enzyme
• Colon Tissue Single-Cell Dissociation Enzyme
• Umbilical Tissue Single-Cell Dissociation Enzyme
• Adipose Tissue Single-Cell Dissociation Enzyme
• Retinal Tissue Single-Cell Dissociation Enzyme
• Ligament Tissue Single-Cell Dissociation Enzyme
• Embryoid Tissue Single-Cell Dissociation Enzyme
• Universal Tissue Single-Cell Dissociation Enzyme

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory