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Cat. No.: HEDRR-50 (for 50ml)

Cat. No.: HEDRR-100 (for 100ml)

Description

The High-Efficiency Debris Removal Reagent is specifically designed for purifying single-cell suspensions prepared from enzymatic digestion or mechanical dissociation of various animal organs (such as brain, liver, spleen, kidney, lung, and tumor tissues). Using a unique gradient separation principle, this reagent rapidly and selectively removes cell debris, dead cells, and impurities, while retaining highly viable and pure target cells (e.g., primary cells, immune cells). It significantly enhances the success rate and data quality of downstream experiments including flow cytometry, single-cell sequencing, and cell culture.

Principle

This reagent is a specially formulated isotonic, sterile solution with precisely optimized density and viscosity. During centrifugation, it forms a stable density barrier:

• High-density/large particles sediment: Cell debris, dead cells (membrane-compromised), and red blood cell aggregates quickly sediment through the barrier to form a pellet.
• Low-density/viable cells remain suspended: Intact, viable target cells with lower density stay above the barrier in the middle-to-upper layer. By simply aspirating this layer, pure viable cells can be efficiently separated from debris.

Features

• Efficient debris removal, purity boost: Eliminates up to 95% of debris and impurities, reducing background noise and improving flow cytometry clarity, sorting yield, and purity.
• Cell-friendly, vitality protection: Gentle separation minimizes damage to viable cells, preserving their native state and biological functions for reliable downstream culture and functional assays.
• Broad compatibility, flexible application: Effective across multiple species (human, mouse, rat) and diverse tissue types.
• Simple operation, time-saving: Classic centrifugation method; no special equipment required. Entire purification process completed within 15 minutes.
• Ready-to-use, stable performance: Strictly quality-controlled, batch-to-batch consistency ensures reproducible results.

Applications

• Pre-treatment for flow cytometry: Removes debris, reduces non-specific background, improves sorting precision.
• Preparation for single-cell RNA sequencing: Provides high-purity, high-viability single-cell suspensions for high-quality sequencing data.
• Primary cell culture and functional studies: Purified cells adhere and grow more effectively, with reduced interference from impurities.
• Functional assays (cytotoxicity, proliferation, apoptosis): Ensures accuracy and reproducibility.
• Isolation and enrichment of rare cells (e.g., stem cells, circulating tumor cells).

Protocol

1. Sample preparation: Resuspend dissociated single-cell suspension in PBS at 1–5 × 10⁶ cells/mL. Mix with reagent at a 1:1 volume ratio; increase reagent proportion if debris is abundant.
2. Centrifugation: Spin at 400–800 × g for 10 minutes (full acceleration/brake enabled). Debris accumulates in the upper layer; intact cells sediment to the bottom.
3. Cell collection: Carefully discard supernatant and resuspend the cell pellet in PBS.
4. Washing/resuspension: Wash cells 1–2 times with PBS or culture medium to obtain high-purity, high-viability target cells for downstream experiments.

Notes

• Debris levels vary by tissue type; optimize reagent-to-suspension ratio through preliminary experiments for best results.
• Supplied sterile; maintain aseptic conditions during use to avoid contamination.
• For research use only.

Related:

• High-Activity Cell Cryopreservation Solution
• Red Blood Cell Lysis Buffer
• High-Efficiency Debris Removal Reagent
• Tissue Single-Cell Dissociation Stop Solution
• High-Activity Tissue Preservation Solution
• Universal Tumor Tissue Single-Cell Dissociation Enzyme
• Pancreas Tissue Single-Cell Dissociation Enzyme
• Skeletal Muscle Tissue Single-Cell Dissociation Enzyme
• Lymph Node Tissue Single-Cell Dissociation Enzyme
• Testis Tissue Single-Cell Dissociation Enzyme
• Thymus Tissue Single-Cell Dissociation Enzyme
• Neonatal Heart Tissue Single-Cell Dissociation Enzyme
• Small Intestine Tissue Single-Cell Dissociation Enzyme
• Colon Tissue Single-Cell Dissociation Enzyme
• Umbilical Tissue Single-Cell Dissociation Enzyme
• Adipose Tissue Single-Cell Dissociation Enzyme
• Retinal Tissue Single-Cell Dissociation Enzyme
• Ligament Tissue Single-Cell Dissociation Enzyme
• Embryoid Tissue Single-Cell Dissociation Enzyme
• Universal Tissue Single-Cell Dissociation Enzyme

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory