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Cat. No.: RA3A-25 (for 25μg)

Cat. No.: RA3A-100 (for 100μg)

Cat. No.: RA3A-500 (for 500μg)

Description

Recombinant APOBEC3A (A3A)—also known as Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 3A, Apolipoprotein B mRNA-editing catalytic polypeptide-like 3A, APOBEC3A, A3A, ARP3, PHRBN, or DNA dC→dU-editing enzyme APOBEC-3A—is a DNA cytosine deaminase.

APOBEC3A is a member of the APOBEC3 gene family and plays a critical role in the host’s antiviral defense. One of its key mechanisms involves deaminating cytosine residues in viral single-stranded DNA (ssDNA), converting them into uracil, which leads to DNA degradation. This enzymatic activity also enables APOBEC3A to introduce C-to-T mutations during PCR amplification, making it a powerful tool for the specific detection of DNA methylation levels.

APOBEC3A is widely used in genome-wide DNA methylation sequencing applications, including Enzymatic Methyl Sequencing (EM-seq) and APOBEC-Coupled Epigenetic Sequencing (ACE-seq).

Traditionally, Whole Genome Bisulfite Sequencing (WGBS) has been considered the gold standard for mapping DNA methylation. However, the harsh chemical reactions involved in bisulfite treatment can damage DNA, causing fragmentation, loss, and GC bias in methylated regions. Enzyme-based methylation sequencing methods offer a gentler alternative that overcomes these limitations.

EM-seq library preparation involves two key enzymatic steps:

1. TET2 (Tet Methylcytosine Dioxygenase 2) and T4-BGT (T4-phage Beta-Glucosyltransferase) are used to convert 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) into 5-carboxylcytosine (5caC) and β-glucosyl-5-hydroxymethylcytosine (5gmC), respectively. These modified bases are resistant to APOBEC3A-mediated deamination.

2. APOBEC3A then selectively deaminates unmodified cytosines (C) to uracils (U), without affecting 5caC or 5gmC, allowing clear differentiation between methylated and unmethylated cytosines.

Together, the combined use of TET2, T4-BGT, and APOBEC3A enables highly specific detection of both 5mC and 5hmC modifications.

Applications

• Catalyzes cytosine deamination to uracil in single-stranded DNA
• Enables high-throughput DNA methylation sequencing, including EM-seq and ACE-seq
• Suitable for enzymatic kinetics studies, inhibitor screening, and selectivity analysis

Source

• Expressed in Escherichia coli using a human APOBEC3A gene construct

Purity

• Free of DNase (endonuclease and exonuclease), RNase, and phosphatase activity
• Purity >95%

Storage 

Store at –20ºC. Stable for at least two years under recommended conditions.

Precautions 

• Keep the product on ice or in an ice bath during use. Return to –20ºC storage immediately after use.
• This product is intended for use by qualified professionals in scientific research only. It is not for use in clinical diagnosis or treatment, food or pharmaceutical applications, and must not be stored in residential settings.
• For your safety and health, please wear a lab coat and disposable gloves when handling this product.

Related:

• Recombinant APOBEC3A (A3A)
• Recombinant Ten-Eleven Translocation 2 (TET2)
• T4 Phage β-Glucosyltransferase (T4-BGT)

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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