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Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

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Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

for Superior Biology Services

  • Home
  • Products 
    • All Products
    • COVID-19
    • Custom Services
    • Catalog Products
    • Innovative Systems
  • About Us 
    • About SBS
    • Achievements-Journals
    • Achievements-Universities
    • Collaboration
    • Legal Statement
    • Contact
  • Ecosystem
  • …  
    • Home
    • Products 
      • All Products
      • COVID-19
      • Custom Services
      • Catalog Products
      • Innovative Systems
    • About Us 
      • About SBS
      • Achievements-Journals
      • Achievements-Universities
      • Collaboration
      • Legal Statement
      • Contact
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Beijing SBS Genetech Co.,Ltd.
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      • PCR-Related​
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    Probe qPCR MasterMix

    Probe qPCR MasterMix

    184.00 - 4,000.00
    Probe qPCR MasterMix is a high-quality premix based on probes for Quantitative PCR (qPCR), which is mainly used for specific ultra-high sensitivity quantitative detection of cDNA or genomic DNA. Since the probes used are generally TaqMan probes, this method is also often called TaqMan assays.
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    All products have special prices for bulk purchase, please contact for more details if required.

     

    Cat. No.: PQM-5 (for 5 ml)

    Cat. No.: PQM-25 (for 25 ml)

    Cat. No.: PQM-250 (for 250 ml)

     

    Description

    Probe qPCR MasterMix uses DNA template and qPCR primers to carry out fluorescence quantitative PCR. It is easy to operate, minimizes human errors, effectively reduces the risk of contamination, saves the operation time of the PCR experiment, and has large detection throughput.

     

    Detection Principle

    Probe-based qPCR uses fluorescent and quencher-labeled DNA probes to target the sequence which will be amplified by PCR. Normally, quenching groups on the probe result in quenching of fluorescent groups due to the fluorescence resonance energy transfer (FRET) in space. When the target sequence is amplified by PCR reaction, both primers and probes are annealed to the target gene. With the extension of primers, the 5' to 3' exonuclease activity of Taq polymerase will cause the probe bound to the target sequence to be degraded gradually from the 5' end. After the fluorescent group and quenching group of the probe are cleaved by Taq polymerase, the quenching group disappears, and the fluorescent group can be normally excited by the excitation light to produce fluorescence. After each PCR cycle, more fluorescent groups are released, and the fluorescence intensity is proportional to the number of newly synthesized target fragments, thus quantitative detection can be achieved. Probes are usually a fragment of linear DNA specific to the target sequence, labeled with fluorescent groups such as FAM or HEX at the 5' end and fluorescent quenching groups such as BHQ1, TAMRA, or MGB at the 3' end.

     

    Features

    • High specificity and sensitivity: Specificity is not only dependent on PCR primers but also specific binding and degradation of probes and target genes to generate fluorescent signals. The detection sensitivity and specificity are usually significantly higher than those of the methods using fluorescent dyes such as SYBR Green.
    • Multiple detections: In a single reaction, different genes correspond to different probes and different probes correspond to different fluorescent markers, which can be used for multiple fluorescent quantitative PCR detection. Probe qPCR MasterMix can be used for the detection of 2-3 genes at the same time after optimization of primers and probes.
    • Inhibition of nonspecific amplification: The polymerase in the mastermix has hot-start property, which can effectively avoid nonspecific amplification caused by nonspecific annealing of primer and template DNA or primer dimer at low temperature.
     

    Storage

    The minimum shelf life is a year at -20°C with light-free. Avoid repeated freeze-thaw cycles.  

     

    Instruction: Protocol

    Related: SYBR Green qPCR Mastermix, Probe One-Step RT-qPCR MasterMix, PrimeTaq™ Probe qPCR MasterMix

     

     

    Only for research and not intended for treatment of humans or animals

     

     

    Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

     

     

    SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

     
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    • Why Choose Us?

    • Reliable Global Supply Chain

      With a reliable global supply chain, we have served top researchers in nearly 60 countries.

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      Our high quality products are trusted by researchers and scientists in top universities and institutes.

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      Powerful Technical Support

      Our support personnel are all experts with average more than 10 years of working experience.

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    • Trusted Supplier

      Thousands of papers have been published in the world's top academic journals with our products.

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    • Cold Spring Harbor Laboratory - SBS Genetech

      SBS Genetech is a long-term sponsor of

      Cold Spring Harbor Laboratory

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