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Cat. No.: PQM-5 (for 5 ml)

Cat. No.: PQM-25 (for 25 ml)

Cat. No.: PQM-250 (for 250 ml)

Description

Probe qPCR MasterMix is a specialized reagent for Real-Time PCR using the Probe hybridization method. It combines DNA polymerase, dNTPs, specific stabilizers, optimized reaction buffer, and other reagents into a 2× Premix reagent suitable for Real-Time PCR detection. It features high sensitivity, strong specificity, and excellent stability.

Probe qPCR MasterMix utilizes the new HM-Taq DNA hot-start polymerase to effectively suppress non-specific PCR amplification, significantly improving amplification efficiency for high-sensitivity Real-Time PCR reactions. HM-Taq DNA polymerase uses an inhibitor to regulate the closure of its substrate binding site through temperature control. At temperatures below 40°C, an inactive enzyme-inhibitor complex is formed. When the temperature rises to the primer-specific annealing temperature, the binding equilibrium shifts towards template-specific primer complex formation. This minimizes the generation of non-specific amplification products throughout the PCR amplification process, greatly enhancing the precision of fluorescence-based quantitative PCR reactions.

Features

• High specificity and sensitivity: Specificity is not only dependent on PCR primers but also specific binding and degradation of probes and target genes to generate fluorescent signals. The detection sensitivity and specificity are usually significantly higher than those of the methods using fluorescent dyes such as SYBR Green.
• Inhibition of nonspecific amplification: The polymerase in the mastermix has hot-start property, which can effectively avoid nonspecific amplification caused by nonspecific annealing of primer and template DNA or primer dimer at low temperature.