SYBR Green qPCR Mastermix is a ready-to-use mastermix that provides superior specificity, robust amplification efficiency, ideal reproducibility, and stability in quantifying target DNA or cDNA, with a minimal level of primer-dimer and non-specific product formation.
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Cat. No.: SGQM-5 (for 5 ml)
Cat. No.: SGQM-10 (for 10 ml)
Cat. No.: SGQM-250 (for 250 ml)
SYBR Green qPCR Mastermix takes advantage of a novel chemically modified hot-start Taq polymerase combined with the antibody. The polymerase activity is inhibited at temperatures below 40°C reversibly. SYBR Green I in the mix will emit fluorescence when binds to the dsDNA amplified in each cycle. This reagent is without ROX Reference Dye in default but can be supplied together with low or high ROX Reference Dye if required. When conducting the experiment, use the SYBR® or SYBR/FAM scan mode setting on the qPCR instrument.
High specificity: Optimized buffer and reversible inhibition can achieve a hot-start effect in the whole reaction.
High efficiency: The Ct value increases earlier than similar products on the market.
High compatibility: This mastermix has been verified on most mainstream qPCR instruments.
The minimum shelf life is 6 months at -20°C with light-free.