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Cat. No.: PRM-2 (for 20 μl × 100)

Cat. No.: PRM-8 (for 20 μl × 400)

Description

Probe One-Step RT-qPCR MasterMix utilizes the TaqMan probe one-step qRT-PCR method. Reverse transcription and qPCR reactions are carried out continuously in the same reaction system. No extra reagent needs to be added during the reaction, which is simple to operate and can effectively prevent contamination. The reverse transcription reaction is conducted by the M-MLV Reverse Transcriptase, which has been removed from the RNase H activity, has high synthesis ability, and is thermal-stable. The reverse transcriptase can perform at a maximum temperature of 60°C, so it has a strong extension ability for RNA templates that have high GC content and complex secondary structure. A DNA polymerase with superior amplification performance is used for qPCR reaction, with higher detection accuracy and sensitivity.

Features

• Use M-MLV Reverse Transcriptase to efficiently generate cDNA from the RNA template, with simple operation steps.
• It has super extension ability for RNA templates with high GC content and secondary complex structure.
• Only specific primers can be used, Oligo (dT) and Random Primer cannot be used.