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Cat. No.: PRCPGC-100 (for 100T) 

Cat. No.: PRCPGC-1K (for 1000T) 

Description

PrimeRT First Strand cDNA Synthesis Premix (5×) contains engineered reverse transcriptase with multi-point mutation by molecular evolutionary technology, which greatly improves the stability and efficiency of reverse transcription. PrimeRT™ First Strand cDNA Synthesis Premix (5×) is an All-in-One tube reverse transcription Mix that contains all the reagents needed for reverse transcription, including engineered reverse transcriptase, RNase inhibitor, random primers, oligo(dT) primer, dNTP mixture, buffer, etc. Only the template RNA and water are needed for the reaction. It makes the synthesis of cDNA much easier and faster, especially suitable for the detection experiment of two-step Real-Time PCR after the synthesis of cDNA.

gDNA Clean can complete genome clearance and reverse transcription reaction at the same time in one step, greatly simplifying the operation and avoiding the risk of contamination and RNA degradation.

Features

• The engineered reverse transcriptase has greatly improved stability and efficiency.
• With this premix, only RNA template and water are required, and the reverse transcription can be completed in 15 min.
• The volume of template RNA can be increased to 80% of the total volume, which is truly suitable for the reverse transcription reaction of low concentration template RNA.
• The premix will not be frozen at -20°C, which reduces the time of thawing and mixing.
• The premix has an optimized ratio of oligo dT and N6 random primers for qPCR detection experiments so that the synthesis of cDNA can start from any regions of RNA transcript with the same efficiency of reverse transcription, which guarantees the repeatability of qPCR to the greatest extent.

Applications

• Conventional PCR
• Real-time PCR 
• Second strand cDNA synthesis
• Construction of cDNA library
• Cloning target gene

Heat Inactivation

Incubation at 85°C for 5 sec. 

Storage

The minimum shelf life is a year at -20°C.

Instruction: Protocol

Related: 

• Viral cDNA 1st Strand Synthesis Kit
• Fast 5-min 1st Strand cDNA Synthesis Kit
• PrimeRT First Strand cDNA Synthesis Premix
• PrimeRT Second Strand cDNA Synthesis Kit
• PrimeRT First Strand cDNA Synthesis Premix (with gDNA Clean)

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory