Ribonuclease R (RNase R) comes from E.coli, obtained by recombinant expression of gene engineering. This enzyme is an Mg2+ dependent 3' - 5' ribonucleic acid exonuclease, which can digest almost all linear RNAs substrates but cannot digest circular RNA, lariat RNA, and double-stranded RNA with 3' protruding ends <7nt. RNase R nuclease exonuclease can efficiently remove linear RNA without secondary structure, so as to purify circular RNA molecules, which is convenient for subsequent RNA sequencing and other experimental studies.
Add to cart
All products have special prices for bulk purchase, please contact us for more details if required.
Cat. No.: ETRHII-200 (for 200U)
Cat. No.: ETRHII-1k (for 1KU)
Extreme Thermostable RNase HII is an endogenous endonuclease derived from extremely thermostable strains. It recognizes RNA/DNA hybrid strands and cleaves RNA strands. It has very low cleavage activity to single-stranded RNA and has no cleavage activity to dsDNA and ssDNA. The RNase HII enzyme cleaves from the 5' end of the single ribonucleotide residue of DNA/RNA duplex to produce a 5' - terminal phosphate group and a 3 '- terminal hydroxyl group. The RNase HII enzyme has the best activity at 70-75°C and is active at 50°C to 75°C, but its activity is very low at room temperature (activity is reduced by~1000 times). The enzyme is extremely heat-resistant and retains all its activity after being heated at 95°C for 15 minutes. Therefore, it is compatible with most PCR buffer systems and can be used in RNase HII dependent PCR (rhPCR) and other experiments. Compared with Rnase HI, Rnase HII can recognize and cut single ribonucleotides, while RNase HI recognizes 2 '- OH of four consecutive ribonucleotides; In addition, Rnase HII can degrade Okazaki fragments, while RNase HI has no such activity.
One unit is defined as the amount of enzyme required to cut 1 nmol of synthetic DNA/RNA hybrid double-stranded substrate containing single rC per minute in 10 mM Tris, 50 mM NaCl, 0.01% Triton X-100, 10 µg/ml BSA, and 4 mM MgCl2 at 70°C.
ET RNase HII (2U/μl): 100 μl
Reduce or remove primer dimer in PCR reaction
SNP detection and rare allele detection
Differentiate heterologous genes
Improve the accuracy of multiplex PCR products
The minimum shelf life is 3 years at -20°C.
When this enzyme is used in rhPCR (RHase HII dependent PCR), it needs to use a DNA primer with a 3' end closure group.
The enzyme is active at 50-75°C, and its dosage can be adjusted according to different applications.
The enzyme is compatible with most PCR buffer systems and has broad requirements for Mg2+concentration. It can play an active role between 1~6 mM.
The enzyme is extremely heat-resistant, and its activity does not decrease significantly at 95°C for 15 minutes, so it cannot be inactivated by heat, but can be inactivated by 0.1% SDS.
Only for research and not intended for treatment of humans or animals
DNA synthesis is a technology that links deoxynucleic acids (adenine, thymine, cytosine, and guanine) together to form DNA. As the cornerstone of modern molecular biology, DNA synthesis plays a pivotal role in the field of synthetic biology. In addition to standard oligos synthesis, we also provide scientific research services such as long oligos synthesis, phosphorothioate oligos (S-Oligo) synthesis, modified oligos synthesis, fluorescent oligos synthesis, and real-time quantitative PCR probes to meet your different needs. Besides, we have been providing high-quality DNA synthesis products (phosphoramidites, controlled pore glass, molecular sieves, etc), and the products have been successfully applied in various fields of molecular biology.
Peptides are synthesized by the condensation reaction of the carboxyl group of one amino acid with the amino group of another amino acid and are widely used in various fields such as antibody preparation, drug development, and peptide vaccine development. Each of our peptides is accompanied by reliable HPLC and mass spectrometry data, detailed synthesis reports are provided, and the products are sent in a lyophilized state. Experienced staff can assist users in designing peptide chains and make appropriate recommendations for different needs of users, such as antibodies, special markers, large-scale synthesis, etc. In addition, we also offer Custom PNA Synthesis of high quality.
Gene synthesis is a technology that synthesizes genes by artificial methods, which is one of the means of gene acquisition. Compared with the acquisition of genes from existing organisms, gene synthesis does not need templates and is therefore not limited by the source of genes. We use unique gene synthesis design software, which includes a full set of tools to design ideal structural units, thus enabling rapid and efficient gene construction and synthesis in a single reaction. Please do not be limited by restriction sites and polylinkers, we will synthesize the various gene sequences you need.
We have been providing high-quality DNA synthesis products (phosphoramidites, controlled pore glass, molecular sieves, etc), and the products have been successfully applied in various fields of molecular biology. The product portfolio includes Synthesis Columns for ABI 3900, MerMade, Dr. Oligos, OligoMaker, ASM-2000, etc., Universal Support-CPG, Standard Support-CPG, DNA Phosphoramidites, LNA Phosphoramidites, Modified Amidites & CPG, Molecular Sieves, etc.
At SBS Genetech, we are at the forefront of offering solutions for isothermal amplification based on our world-class platform. Our Bst DNA/RNA Polymerase is at the core of this platform, which is a mixture of Bst polymerase and extremely thermostable reverse transcriptase. Based on this special enzyme, we have developed PrimeIampTM lyophilized isothermal amplification microbeads series. These series are ready-to-use master mixes, which can perform isothermal amplification directly when the templates and primers are added. With freeze-drying technology, these master mixes are lyophilized into solid microbeads, which can be transported and stored at room temperature with great convenience.
GoodView™ is a safer nucleic acid stain, an alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 268 nm and the other at 294 nm. In addition, it has one visible excitation at 491 nm. The Fluorescence emission of GoodView bound to DNA is centered at 530 nm.Our GoodView™ Nucleic Acid Stain is included in New Products, Science Journal, January 11, 2019.
Polymerase chain reaction (PCR) is a widely used method in molecular biology, which can rapidly replicate millions to billions of specific DNA samples, enabling scientists to extract only a small amount of DNA samples for detailed research. We provide a variety of DNA polymerases (Taq, Bst, Pfu) and corresponding PCR premixes, covering a wide range of scenarios such as high fidelity, high specificity, and rapid amplification. High-quality dNTPs and NTPs (set, mix) are also supplied.
Ribonucleic acid (RNA), as a key material for genetic information transmission and cell regulation, has been extensively studied in molecular biology. Like DNA, RNA is assembled in the form of nucleotide chains; but unlike DNA, RNA exists in nature in the form of single-stranded folds rather than paired double strands. We provide high-quality NTPs, RNasin (RNase inhibitor), and M-MLV reverse transcriptase to provide a complete raw material solution for RNA research. miAnalysis™ series are designed for microRNA quantitative research. And VirusMag™ series is designed for the isolation of viral RNA/DNA or bacterial DNA.
RNA silencing technology has become a powerful tool to study gene function. The success of any RNA experiment depends on high-quality siRNA and effective transfection reagent. With chemical modification, our chemically modified siRNA has much higher stability than the common siRNA. The chemical modification not only enhances the life span of siRNA in serum and cell culture but also enhances its activity in vitro. As for transfection reagent, our ready-to-use siRNA transfection reagent, Sirnafectamine, can be used for a wide range of cell lines, with minimal cytotoxicity and the best cell state after transfection. As Sirnafectamine will protect RNA during the whole process, a very low concentration of siRNA can produce high gene silencing efficiency.
Nucleic acid purification is an important component of molecular biology and has a wide range of applications in medicine and biological sciences. Our nucleic acid purification kit uses first-class silica gel column technology (SiMax™ Spin Column) and magnetic beads technology (VSep™ Magnetic Separators, VirusMag™ One-Step DNA/RNA Isolation Kit, VirusMag™ DNA/RNA Isolation Kit), which can purify DNA/RNA from various sources quickly and reliably. The purity of DNA/RNA purified by our kit is very high and it is suitable for many downstream applications such as sequence determination, cloning, and cleavage.
Microspheres are small spherical beads formed by polymer polymerization through nanotechnology, which are widely used in the field of in vitro diagnosis. We are at the forefront of providing various types of microspheres with high quality and batch-to-batch consistency, which builds a solid foundation for large-scale production. Our products include magnetic microspheres, latex microspheres, dyed microspheres, fluorescent microspheres, flow cytometry microspheres, and standard microspheres.
DNA markers are used to determine the approximate size of molecules on the gel during electrophoresis, based on the principle that the molecular weight is inversely proportional to the mobility through the gel matrix. We provide abundant DNA molecular weight standards that can be used for various fragment lengths. Our fragments of DNA markers have been purified separately by proprietary technology, so their quality is superior to industry standards.
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria and archaea. The gene-editing technology based on this system has a wide variety of applications. Here, we provide various Cas nucleases, synthetic sgRNAs, and T7 Endonuclease I of high quality to improve the accuracy and efficiency of your experiments. We also offer the Cas13a Nuclease (Lyophilized) and Cas12a Nuclease (Lyophilized) that can be transported at room temperature, saving the high cost of dry ice transportation.