Ribonuclease H ( RNase H ) is an endo-ribonuclease, which can specifically degrade the phosphodiester bond of RNA hybridized to the DNA strand, so it can degrade the RNA strand in the RNA / DNA hybrid strand. This enzyme cannot digest single- or double-stranded DNA. This RNase H enzyme is widely used to remove mRNA poly (A) hybridized to poly (dT) or to remove mRNA during second-strand synthesis of cDNA.
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Cat. No.: RNH-250 (for 250U)
Cat. No.: RNH-2500 (for 2,500U)
Unit definition One unit refers to the amount of enzyme required to hydrolyze 1 nmol of a nucleic acid substrate from a 40 pmol RNA-DNA hybrid strand of 25 bp in length in a 50 μl reaction system at 37°C within 20 minutes.
1 × Rnase H Buffer: 75 mM KCl, 50 mM Tris-HCl pH 8.3, 3 mM MgCl2, 10mM DTT.
The optimal reaction temperature of the enzyme is 37°C, and the enzyme is inactivated at 65°C for 20 min.
Store the components at -20°C. They are stable for three years under suggested storage conditions. Avoid multiple freeze-thaw cycles and exposure to frequent temperature changes.
Only for research and not intended for treatment of humans or animals
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