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Cat. No.: DFRAS-20 (for 20T)

Cat. No.: DFRAS-100 (for 100T)

Cat. No.: DFRAS-500 (for 500T)

Description

DNA-free RNA Sample Preparation Kit with Agarose (Salt-tolerant), also known as DNA Removal Kit, DNA-free Kit, DNA Salt-tolerant Removal Kit, DNA Residue Removal Kit, or DNase Treatment and Removal Reagents, is a specialized kit designed to efficiently eliminate DNA contamination from RNA samples using high-quality DNase I-XT. It further removes DNase I-XT and divalent cations through the use of DNase Inactivation Agarose, ensuring high purity of RNA samples. RNA prepared with this kit is free of residual DNA and suitable for downstream applications such as RT-qPCR, RT-PCR, RNA Sequencing, RNA Microarray, Ribonuclease Protection Assays (RPAs), Northern blotting, and other RNA analyses.

This kit offers comparable performance and workflow to Invitrogen’s TURBO DNA-free™ Kit (AM1907).

RNA extracted using conventional methods often contains trace amounts of DNA contamination, especially when isolated from tissues such as the spleen, thymus, or kidney. Such contamination can interfere with subsequent RNA analyses. This kit includes Recombinant DNase I-XT (2 U/μl), Reaction Buffer (10X), DNase Inactivation Agarose, and Nuclease-free Water, enabling effective removal of residual DNA and the introduced DNase I-XT from RNA samples.

Components

• Recombinant DNase I-XT (2U/μl)

• Reaction Buffer (10X)

• DNase Inactivation Agarose

• Nuclease-free Water

Features

The DNA-free RNA Sample Preparation Kit with Agarose provides high-activity, high-purity DNase I-XT with excellent performance. The included Recombinant DNase I-XT (2 U/μl) is a salt-tolerant DNase I variant, expressed via Pichia pastoris and engineered through protein modification. It offers high purity, strong enzymatic activity, and is free from animal-derived components and RNase contamination.

This kit enables rapid, specific, and efficient removal of DNase I-XT with a streamlined workflow. After adding DNase Inactivation Agarose to the reaction mixture and incubating at room temperature for 5 minutes, a brief centrifugation at 10,000 × g for 90 seconds effectively removes DNase I-XT. This eliminates the need for traditional inactivation methods such as 65ºC heat treatment, phenol/chloroform extraction, ethanol precipitation, or EDTA chelation. The removal efficiency of DNase I-XT is comparable to leading international products.

The kit also effectively removes divalent cations, minimizing RNA degradation and preserving sample integrity and recovery. At temperatures above 60ºC, ions such as Mn²⁺, Zn²⁺, Cu²⁺, Mg²⁺, and Ca²⁺ can accelerate RNA degradation. Since the Reaction Buffer used with DNase I-XT contains Mg²⁺ and Ca²⁺, failure to remove these ions may compromise RNA stability in downstream applications. The DNase Inactivation Agarose in this kit binds divalent cations efficiently, ensuring that purified RNA remains intact, minimally damaged, and highly recoverable.

Storage

Store at –20ºC. Valid for one year.

Precautions

• During use, Recombinant DNase I-XT (2 U/μl) should be kept on ice or in an ice bath, and promptly returned to –20ºC storage after use.

• All procedures must be conducted under RNase-free conditions. Wear masks and gloves to minimize contamination from RNases present on the human body. Avoid breathing, speaking, or exhaling directly over samples, reagents, or consumables to prevent RNase contamination.

• All reagents and consumables used with this kit must be RNase-free. Handle with care to avoid contamination. If consumables are suspected of RNase contamination, soak them overnight in 0.01% DEPC-treated water, followed by autoclaving and drying.

• Although DNase Inactivation Agarose may also remove other protein contaminants, if the RNA sample contains high levels of protein impurities, it is recommended to purify the RNA beforehand to reduce protein contamination.

• This product is intended solely for scientific research by professionals. It must not be used for clinical diagnosis or treatment, not be used in food or pharmaceuticals, and must not be stored in residential settings.

• For your safety and health, please wear a lab coat and disposable gloves during operation.

Related:

We also offer the following DNA-free RNA Sample Preparation Kits based on different purification methods:

• Agarose Gel Method: DNA-free RNA Sample Preparation Kit with Agarose

• Salt-Tolerant DNase/Agarose Gel Method: DNA-free RNA Sample Preparation Kit with Agarose (Salt-tolerant)

• Magnetic Agarose Bead Method: DNA-free RNA Sample Preparation Kit with Magnetic Agarose Beads

• Salt-Tolerant DNase/Magnetic Agarose Bead Method: DNA-free RNA Sample Preparation Kit with Magnetic Agarose Beads (Salt-tolerant)

Among these, Salt-Tolerant DNase I-XT demonstrates significantly higher salt resistance compared to conventional DNase I. When the salt concentration exceeds 50 mM, the activity of standard DNase I is markedly inhibited, often resulting in incomplete DNA degradation. In contrast, DNase I-XT exhibits optimal activity at salt concentrations between 50–150 mM, and retains approximately 60% activity even at 350 mM, making it highly effective under high-salt conditions.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory