All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: DEMAB-10 (for 10ml, 25%(v/v))

Cat. No.: DEMAB-50 (for 50ml, 25%(v/v))

Cat. No.: DEMAB-200 (for 200ml, 25%(v/v))

Description

DEAE Magnetic Agarose Beads, also known as DEAE Anion Exchange Agarose Magnetic Beads, DEAE Anion Exchange Beads, DEAE Weak Anion Exchange Beads, DEAE Protein Purification Magnetic Beads, DEAE Ion Exchange Magnetic Agarose Beads, or DEAE Ion Exchange Beads, are weak anion‑exchange magnetic beads formed by covalently coupling diethylaminoethyl (DEAE) groups to agarose magnetic beads. These beads can rapidly, efficiently, sensitively, and specifically bind negatively charged biomacromolecules, and are mainly used for the separation and purification of negatively charged natural or recombinant proteins, antibodies, peptides, and nucleic acids.

Ion‑exchange chromatography (IEC) is an effective method for separating and purifying biomolecules. It exploits differences in the type and magnitude of charges carried by biomolecules under specific conditions, achieving separation through electrostatic interactions between oppositely charged species. Ion‑exchange media consist of three components:

1. Matrix – a cross‑linked polymer backbone such as agarose, dextran, or cellulose gel.

2. Ligand – the charged functional group covalently attached to the matrix. The ligand determines the properties of the ion‑exchange medium. Ligands with negative charges form cation‑exchange media, which bind positively charged proteins; ligands with positive charges form anion‑exchange media, which bind negatively charged proteins.

3. Counter ion – an ion with the opposite charge of the ligand, which can reversibly associate with the ligand and maintain charge balance.

Our ion‑exchange agarose magnetic bead series includes:

• SP Agarose Magnetic Beads (strong cation exchanger)

• CM Agarose Magnetic Beads (weak cation exchanger)

• Q Agarose Magnetic Beads (strong anion exchanger)

• DEAE Agarose Magnetic Beads (weak anion exchanger)

A comparison and selection guide for these four types of agarose magnetic beads is provided in the table below.

This product is an anion‑exchange magnetic bead in which DEAE (diethylaminoethyl) serves as the functional group and agarose magnetic beads serve as the matrix. DEAE is a weakly basic tertiary amine group with excellent stability. The central nitrogen atom carries a positive charge, enabling it to adsorb negatively charged proteins and other biomolecules in the sample. These negatively charged molecules are retained on the beads and can then be eluted by increasing the salt concentration or adjusting other elution conditions, thereby achieving efficient purification of negatively charged biomacromolecules.

DEAE agarose magnetic beads are widely used in the biomedical field. They can specifically purify proteins, peptides, antibodies, and other downstream products from biopharmaceutical or bioengineering processes. They are also suitable for separating polysaccharides from cells and plants, particularly neutral, acidic, and mucopolysaccharide substances. In addition, they can be used to isolate large amounts of genomic DNA as well as plasmid DNA from bacterial or cellular lysates.

This product enables rapid and efficient separation through magnetic adsorption, eliminating the need for centrifugation.

Features

• This product has a high binding capacity. Compared with many similar products, it offers an exceptionally high binding capacity, enabling rapid purification of negatively charged proteins, polysaccharides, nucleic acids, and other biomolecules from complex samples. Each milliliter of DEAE agarose bead sediment can bind approximately 0.14–0.18 mmol of Cl⁻, corresponding to about 50 mg of BSA.
• This product provides high specificity. It specifically binds negatively charged proteins, peptides, polysaccharides, and nucleic acids, yielding highly pure products suitable for downstream applications such as Western blotting, ELISA, mass spectrometry, and other analytical assays.
• This product is easy to use. It is stored in a special protective solution without glycerol and enables rapid and efficient separation through magnetic adsorption, eliminating the need for centrifugation.
• DEAE Agarose 6FF is also available.
• This product is supplied as a 25% agarose magnetic bead suspension, with the package volume representing the total suspension volume. Each milliliter of product contains 0.25 mL of agarose bead sediment.

Storage

Store at 4ºC with a two‑year shelf life.

Precautions

• This product should be maintained at pH 6–8 and should not be subjected to high‑speed centrifugation or drying. Do not leave the magnetic beads in a magnetic field for extended periods, as this may cause bead aggregation.
• It is recommended to include both positive and negative controls during purification.
• Protein samples should be purified as soon as possible after collection and kept at 4ºC or on ice at all times to slow protein degradation or denaturation. To effectively inhibit protein degradation, an appropriate amount of protease inhibitor cocktail may be added to the sample.
• If centrifugation does not completely remove insoluble material from the protein sample, the solution may be filtered through a 0.45 μm membrane.
• When using a vacuum pump or similar device to remove supernatant, pay attention to the suction strength to avoid accidentally aspirating aggregated magnetic beads.
• A 0.1% non‑ionic detergent (such as Triton X‑100, Tween‑20, or NP‑40) can effectively prevent bead aggregation without affecting the protein‑binding efficiency of the beads.
• High concentrations of DTT, β‑mercaptoethanol, and similar reducing agents may affect the interaction between this product and target molecules. This product is also incompatible with cationic detergents such as CTAB or cetylpyridinium chloride, as well as oxidizing agents.
• This product is intended for research use only by trained professionals. It is not for clinical diagnosis or treatment, not for use in food or pharmaceuticals, and should not be stored in residential settings.
• For your safety and health, please wear a lab coat and disposable gloves during operation.

Related:

• DEAE Magnetic Agarose Beads
• Q Magnetic Agarose Beads
• CM Magnetic Agarose Beads
• SP Magnetic Agarose Beads

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory