• Recombinase Polymerase Amplification (RPA) System

    Rapid, sensitive, and instrument-free nucleic acid amplification

    SBS Genetech provides next-generation RPA solutions with engineered enzymes, multiplex capabilities, and lyophilized formats for advanced molecular detection workflows

  • What is Recombinase Polymerase Amplification (RPA)?

    A rapid, low-temperature isothermal amplification technology enabling sensitive nucleic acid detection without thermal cycling

    Recombinase Polymerase Amplification (RPA) is a rapid and highly sensitive isothermal nucleic acid amplification technology that enables DNA and RNA detection at constant temperatures, typically between 37°C and 42°C. Unlike conventional PCR, which requires repeated temperature cycling, RPA amplifies target nucleic acids under simple, low-temperature conditions without the need for complex thermal cyclers.
    By combining the activities of recombinase, single-stranded DNA binding proteins (SSB), and strand-displacing DNA polymerase, RPA enables efficient target amplification within minutes. This unique mechanism makes RPA particularly suitable for applications requiring rapid turnaround time, minimal instrumentation, and decentralized testing.
    Today, RPA has become a powerful molecular detection platform for point-of-care testing (POCT), infectious disease diagnostics, food safety testing, veterinary applications, environmental monitoring, and research workflows.

  • How Does RPA Work?

    RPA achieves rapid amplification through a unique enzyme-driven mechanism that mimics natural DNA replication processes

    1
    Recombinase-Primer Complex Formation

    Recombinase proteins bind primers and facilitate their invasion into homologous target DNA sequences.

    2
    Strand Separation and Target Recognition

    The recombinase-primer complex searches for complementary sequences and initiates strand exchange without high-temperature denaturation.

    3
    DNA Extension

    A strand-displacing DNA polymerase extends the primers, generating new DNA products while continuously amplifying the target sequence.

    4
    Signal Generation

    The amplified products can be detected through fluorescence, lateral flow assays, real-time monitoring, or integrated CRISPR-based detection systems.

  • Why Choose RPA?

    Combining rapid amplification, low-temperature operation, and flexible detection formats, RPA enables a new generation of simple, sensitive, and decentralized molecular testing solutions

    Rapid Results

    RPA can generate detectable amplification signals within 10–20 minutes, enabling faster molecular testing compared with conventional PCR workflows.

    Low Temperature Operation

    RPA operates at 37–42°C, allowing amplification using simple heating devices and portable platforms.

    Minimal Instrument Requirements

    Without thermal cycling, RPA can be integrated into decentralized and field-based molecular testing systems.

    Flexible Detection Formats

    RPA products can be combined with fluorescence, lateral flow, and CRISPR-based detection technologies.

  • Powering Next-Generation RPA with a Complete Enzyme Toolkit

    Achieving rapid, sensitive, and reliable isothermal amplification through optimized enzyme systems, integrated reaction chemistry, and stabilized lyophilized RPA formats

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    Our core offering is a lyophilized Recombinase Polymerase Amplification (RPA) kit, designed for rapid, isothermal nucleic acid amplification of DNA and RNA targets. The freeze-dried formulation integrates all essential reaction components into a single, stable format, enabling simplified molecular diagnostic workflows, improved reagent stability, and easy transport for decentralized and field-based applications.

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    To ensure robust assay performance and flexibility, our RPA system is supported by a comprehensive enzyme platform, including key functional proteins such as T4 UvsX Recombinase, T4 UvsY Protein, T4 Gene 32 Protein, Bsu DNA Polymerase, Sau DNA Polymerase, Exonuclease III, Bst DNA/RNA Polymerase, and Endonuclease IV, which collectively enable efficient strand invasion, DNA synthesis, and reaction control in isothermal amplification systems.

  • Evolution of our RPA System

    1

    Gen-1 RPA System

    Highly stable amplification system

    2

    Gen-2 RPA System

    Introduction of an additional Sucrose-Sucrose Phosphorylase Energy System, making the amplification system even more stable and reliable.

    3

    Gen-3 RPA System

    Introduction of an additional dUTP-UDG-UGI System to effectively prevent aerosol contamination.

    4

    Gen-4 RPA System

    Revolutionary Exclusive Multiplex Recombinase Polymerase Amplification (RPA) Kit Capable of Completing Direct Amplification of DNA/RNA within 10 to 20 Minutes.

    5

    CRISPR + RPA System

    Integrating Cas12a/Cas13a detection with RPA amplification enables a novel diagnostic approach that combines rapid signal generation, high sensitivity, and ease of use, paving the way for next-generation point-of-care testing technologies.

  • General Guide for RPA Primer & Probe Design

     

  • RPA System Portfolio

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    CRISPR Cas12b-RT-LAMP RNA Lyophilized Detection Kit (One-Step)
    $616.00 - $2,320.00
    $2,900.00
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    CRISPR Cas12b-LAMP DNA Lyophilized Detection Kit (One-Step)
    $528.00 - $1,980.00
    $2,475.00
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    RPA Multiplex Basic Lyophilized Kit (Gen-4)
    $1,276.00 - $8,294.00
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    RPA Multiplex Fluorescent Lyophilized Kit (Gen-4)
    $1,276.00 - $8,294.00
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    Lateral Flow Assay for RPA
    $395.00 - $695.00
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    CRISPR Cas13a-RPA RNA Lyophilized Detection Kit (Two-Step) (Test Strip)
    $1,900.00 - $6,300.00
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    CRISPR Cas13a-RPA RNA Lyophilized Detection Kit (Two-Step)
    $1,250.00 - $4,400.00
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    CRISPR Cas13a-RPA RNA Lyophilized Detection Kit (One-Step)
    $1,700.00 - $5,900.00
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    CRISPR Cas12a-RPA RNA Lyophilized Detection Kit (Two-Step) (Test Strip)
    $1,360.00 - $4,500.00
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    CRISPR Cas12a-RPA RNA Lyophilized Detection Kit (Two-Step)
    $1,245.00 - $4,180.00
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    CRISPR Cas12a-RPA RNA Lyophilized Detection Kit (One-Step)
    $1,250.00 - $4,200.00
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    CRISPR Cas13a-RPA DNA Lyophilized Detection Kit (Two-Step) (Test Strip)
    $1,800.00 - $5,950.00
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    CRISPR Cas13a-RPA DNA Lyophilized Detection Kit (Two-Step)
    $1,185.00 - $4,150.00
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    CRISPR Cas13a-RPA DNA Lyophilized Detection Kit (One-Step)
    $1,600.00 - $5,600.00
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    CRISPR Cas12a-RPA DNA Lyophilized Detection Kit (Two-Step) (Test Strip)
    $1,285.00 - $4,250.00
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    CRISPR Cas12a-RPA DNA Lyophilized Detection Kit (Two-Step)
    $1,175.00 - $3,950.00
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    CRISPR Cas12a-RPA DNA Lyophilized Detection Kit (One-Step)
    $1,185.00 - $4,150.00
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    DNA-Free E. coli Endonuclease III (Nth)
    $320.00 - $1,280.00
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    DNA-Free Sau DNA Polymerase (Large Fragment)
    $360.00 - $1,440.00
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    DNA-Free T4 Gene 32 Protein
    $228.00 - $2,964.00

  • Trusted by Researchers Worldwide

    SBS Genetech products have supported thousands of peer-reviewed publications across molecular biology, diagnostics, genomics, synthetic biology, and life science research

    Journals Using SBS Genetech Products: Science, Cell, Cancer Cell, Cell Metabolism, Immunity, Nucleic Acids Research, Advanced Science, etc.

    Journals Using SBS Genetech Products

    Universities Using SBS Genetech Products: Harvard University, Imperial College London, National University of Singapore, Cornell University, Tsinghua University, etc

    Universities Using SBS Genetech Products

  • Published Papers

    Representative Publications Using SBS Genetech Isothermal Amplification Products

     

    Xue, T. , Ma, Z. , Liu, F. , Du, W. , & An, C. . (2020). Pneumocystis jirovecii colonization and its association with pulmonary diseases: a multicenter study based on a modified loop-mediated isothermal amplification assay. BMC Pulmonary Medicine, 20(1).

     

    Zhao, K. , Hu, R. , Ni, J. , Liang, J. , & Li, C. . (2020). Establishment of a porcine parvovirus (PPV) LAMP visual rapid detection method. Journal of Virological Methods, 284, 113924.

     

    Papadakis, G. , Pantazis, A. K. , Fikas, N. , Chatziioannidou, S. , Tsiakalou, V. , & Michaelidou, K. , et al. (2022). Portable real-time colorimetric LAMP-device for rapid quantitative detection of nucleic acids in crude samples. Scientific Reports, 12(1), 1-15.

     

    Wu, R. , Liu, X. , Guo, B. , Chen, F. , & Wang, X. . (2014). Development of double loop-mediated isothermal amplification to detect listeria monocytogenes in food. Current Microbiology, 69(6), 839-845.

     

    Ji, J. , Du, L. Q. , Xie, Q. M. , Cao, Y. C. , & Bee, Y. Z. . (2009). Rapid diagnosis of duck plagues virus infection by loop-mediated isothermal amplification. Research in Veterinary Science, 87(1), 53-58.

     

    Zhang, S. , Xu, X. , Wu, Q. , & Zhang, J. . (2013). Rapid and sensitive detection of pseudomonas aeruginosain bottled water by loop-mediated isothermal amplification. European Food Research & Technology, 236(1), 209-215.

     

    Xu, X. , Zhang, S. , Wu, Q. , Zhang, J. , Li, F. , & Cheng, J. . (2014). Development and Application of a Loop-Mediated Isothermal Amplification (LAMP) Method for Rapid and Sensitive Detection of Enterococcus faecalis in Drinking Water. Journal of Food Safety, 34(2), 103–110.

     

    Zhang, F. , Shi, Y. , Jiang, K. , Song, W. , Ma, C. , & Xu, Z. , et al. (2014). Rapid detection and quantification of Prorocentrum minimum by loop-mediated isothermal amplification and real-time fluorescence quantitative PCR. Journal of Applied Phycology, 26(3), 1379-1388.

  • More Information

  • Contact

    For more information, please fill out the provided form or contact us directly by E-mail

    Room 11E, Building 2
    No.2 Shangdi Information Road
    Haidian District
    Beijing 100085 China
    (0086-10)62969345/46
    (0086-10)62969345/46
    tech@sbsbio.com
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