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Exonuclease III
Exonuclease III was derived from E.coli, with 3 '- 5' exonuclease activity, acts on double-stranded DNA, and gradually cuts single nucleotides from the 3 'OH terminal direction. Each time the enzyme binds to the substrate for catalysis, only a few nucleotides are removed, resulting in progressive deletion within the DNA molecule group.
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Cat. No.: EXN3-10k (for 10KU)
Cat. No.: EXN3GF-10k (for 10KU, glycerol free)
The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system.
Description
Exonuclease III was derived from E.coli, with 3 '- 5' exonuclease activity, acts on double-stranded DNA, and gradually cuts single nucleotides from the 3 'OH terminal direction. Each time the enzyme binds to the substrate for catalysis, only a few nucleotides are removed, resulting in progressive deletion within the DNA molecule group.
The best substrate of this enzyme is the flat end or 5' protruding end double-stranded DNA molecule, but it can also act on the notch of double-stranded DNA, degrading DNA molecules from 3', and releasing 5' single nucleotides. For the 3' protruding end, especially those more than 4nt, it almost does not cut at all, nor can it cut the thiophosphoryl lipid bond.
The activity of Exonuclease III depends partly on the helical structure and varies according to the sequence (C > A = T > G). In addition, Exonuclease III also has de-purine / pyrimidine endonuclease activity, RNaseH activity, and 3' phosphatase activity.
Applications
Nondirectional nested deletion
Preparation of single strand bottom template for dideoxy sequencing
Preparation of strand-specific probes
Site-directed mutagenesis
Unit Definition
One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble product in 50 μl reaction system at 37°C for 30 minutes with 0.15 mM sonicated duplex [3H]-DNA.
Storage
The minimum shelf life is 3 years at -20°C. Avoid repeated freezing and thawing.
Reagents for Recombinase Polymerase Amplification (RPA)
- T4 UvsX Recombinase
- T4 UvsY Protein
- T4 Gene 32 Protein
- Bsu DNA Polymerase
- Sau DNA Polymerase
- Exonuclease III
- Bst DNA/RNA Polymerase
- Endonuclease IV
Instruction: RPA Reaction System (for reference only)
RPA Isothermal Amplification Kits
In addition to the RPA raw material enzymes mentioned earlier, we offer a diverse selection of kits designed to meet your specific needs. Explore our exceptional offerings:
- RPA DNA Amplification Reagent Kit (Basic Type)
- RPA DNA Amplification Reagent Kit (Exol Ⅲ Fluorescence Type)
- RPA RNA Amplification Reagent Kit (Basic Type)
- RPA RNA Amplification Reagent Kit (Exol Ⅲ Fluorescence Type)
- RPA DNA Rapid Amplification Reagent Kit (ExolII Fluorescence Enhancement Type)
- RPA RNA Rapid Amplification Reagent Kit (ExollI Fluorescence Enhancement Type)
- Influenza A Virus Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
- Influenza B Virus Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
- 2019-nCoV Nucleic Acid (N) Detection Kit (Isothermal Amplification Fluorescence Method)
- 2019-nCoV Nucleic Acid (N) Detection Kit (Isothermal Amplification CRISPR Method)
- African Swine Fever Virus Nucleic Acid Detection Kit (Isothermal Amplification CRISPR Method)
- White Spot Syndrome Virus Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
- Monkeypox Virus Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
- Adenovirus Type 3 Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
- Adenovirus Type 7 Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
- Streptococcus Pyogenes Nucleic Acid Detection Kit (Isothermal Amplification Fluorescence Method)
Our kits are meticulously crafted to deliver accurate and efficient results for your research endeavors. For further information or if you're interested in any of our products, please don't hesitate to contact us. Elevate your work with our cutting-edge solutions today!
Only for research and not intended for treatment of humans or animals
